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1、中国农业科学2011,44(23):4807-4814ScientiaAgriculturaSinicadoi:10.3864/j.issn.0578-1752.2011.23.006枯草芽胞杆菌Bs-916的全基因组分析王晓宇，罗楚平，陈志谊，刘永锋，刘邮洲，聂亚锋，于俊杰，尹小乐（江苏农业科学院植物保护研究所，南京210014）摘要：【目的】对枯草芽胞杆菌Bs-916进行全基因组测序，为今后更好挖掘和利用该菌生防潜力提供较多的分子生物学信息。【方法】通过应用比较基因组学软件与另一测序菌株Bs168进行全基因组序列分析。【结果】Bs-916菌株全基因组大小为39259
2、58bp，GC含量为46.4%，与其它已测序全基因组芽孢杆菌相比，其GC含量最高；预测所得CDS数为4056个，152个串联重复区域，转座子103个，IS（插入序列）序列数量37个，tRNA46个，rRNA39个；比较基因组学分析其含有8个NRPS/PKS基因簇，其中bacillomycinL，macrolactin，difficidin这3个基因簇在比较菌株Bs168中不存在；该菌还含有植酸酶基因、comAPQX及sfp等与生防因子相关的基因。【结论】Bs-916菌株全基因组含有多种抗菌物质编码基因簇，是一类具有极大生防潜力的典型根际革兰氏阳性菌株。该菌株全基因组序列
3、的测定及其遗传操作方法的可行性，有利于其次生代谢产物的开发和利用。次生代谢产物具有潜在的应用价值，有望在未来开发成独特的农业生物工程制剂。关键词：枯草芽孢杆菌；比较基因组学；共线性；GC偏好TheCompleteGenomeSequenceoftheGram-PositiveBacteriumBacillussubtilsBs-916WANGXiao-yu,LUOChu-ping,CHENZhi-yi,LIUYong-feng,LIUYou-zhou,NIEYa-feng,YUJun-jie,YINXiao-le(InstituteofPlantProtection,J
4、iangsuAcademyofAgriculturalSciences,Nanjing210014)Abstract:【Objective】TheobjectiveofthisstudyistosequencethewholegenomeofBacillussubtilsBs-916andprovidemoreinformationaboutmolecularbiologyforfutureminingandutilizingthepotentialofthisstrain.【Method】Applicationofcomparativegenomicssoftware
5、s,thewholegenomesequenceanalysiswascarriedoutwithBs168strains.【Result】Bs-916strainsgenomeis3925958basepairscomprises4056protein-codinggenes.TheaverageGCratiois46.4%,thehighestcomparedwithotherwhole-genomesequencingofBacillusspp..Itcontains152tandemrepeatregion,103transposons,37IS(Inserts
6、equence),46tRNA,39rRNA.Throughcomparativegenomicsanalysis,Bs-916harborseightgiantgeneclustersdirectingsynthesisofbioactivepeptidesandpolyketidesbymodularlyorganizedmega-enzymesnamednon-ribosomalpeptidesynthetases,NRPSandpolyketidesynthases,PKS.Macrolactin,difficidin,andbacillomycinLareab
7、sentinBs168strains.Bs-916alsocontainsphytasegene,comAPQXandsfprelatedwithbiocontrolmechanism.【Conclusion】Bs-916straingenomecontainsmanygeneclusterencodingavarietyofantimicrobialsubstances,itcanbeconsideredaparadigmforanowngroupofplant-associatedgram-positivebacteriawithah
