优质型肉鸡品系(s3系)mstn、ppar和lpl基因多态性与屠宰及肉质性状相关性研究

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1、优质型肉鸡品系(S3系)IllSTN,PPAR禾FJ￡忍基因多态性与屠宰及肉质性状相关性研究StudiesonassociationsbetweenpolymorphismofMSTN,PPARandLpLGeneandMeatQualityTraitsinBroilers(S3Strain)现代农业产业技术体系建设专项(CARS一42一Z06)；扬州市农业科技攻关项目(YZ2010050)资助扬州大学动物科学与技术学院2013年5月优质型肉鸡品系(S3系)MSTN,PPARLPL基因多态性与屠宰及慧兰芝嚣性研究～煳嬲研究生：温彦涛1V岑。省

2、公。燮巴必幽f中文摘要IIStudiesonassociationsbetweenpolymorphismofMSTN,即4RandLpLGeneandMeatQualityTraitsinBroilers(S3Strain)M．S．Candidate：YantaoWenSupervisor：Prof．Xing·longWangMajor：AnimalGeneticsandBreeding(CollegeofAnimalScienceandTechnology,YangzhouUniversity,225009，Yangzhou)Abstra

3、ctInordertoobtainsuitablemoleculargeneticmarkerswhichprovideatheoreticalbasisofmolecularmarker-assistedselectionforQualitybroilerS3strain．MSTNgene、P助尺geneandLPLgenewereselectedascandidategenesaffectingcorrelatetraitsinQualitybroilers．PCR-RFLPandPCR-SSCPwereusedtodetecttheSN

4、Psincandidategenesinordertoresearchthecorrelationbetweendifferentgenotypesandslaughtertraits&meatqualitytraits．Theresultsaleasfollows：1．TwoSNPswerefoundattheexon1ofMSTNgene．G—Cmutationatthepositionof195bpandA—Gmutationat234bpwhichcausesnochangesofaminoacid．Associationwithsl

5、aughter：atG195Cposition，genotypeCCweresignificantlyhigherthanGGinSemievisceratedPercentage，EvisceratedPercentage，BreastMuscleWeightandThighMuscleWeight(P<0．05)；atA234Gposition，genotypeGGweresignificantlyhigherthanAGinSemievisceratedPercentage，EvisceratedPercentage(P<0．05)in

6、cocks．ItsuggestedthattheMSTNgenewasafunctionalgeneofaffectingtheslaughtertraits，couldbeusedasmolecularmarkersofslaughtertraitsinS3broilers．2．ASNPwerefoundinexon6ofPPARgene．A_Gmutationatthepositionof71438078bp．Associationwithmeatquality：WaterHoldingCapacityingenotypeCTweresi

7、gnificantlyhigherthanCC(P

8、formeatqualitytraits．ASNPwerefoundinintrons10，A_÷Gmutationatthepositionof54352843b