异丙酚预处理对大鼠两种急性呼吸窘迫综合征模型的保护作用比较

《异丙酚预处理对大鼠两种急性呼吸窘迫综合征模型的保护作用比较》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、贵阳医学院2013届硕士研究生论文《中国图书资料分类法》单位代码：10660分类号：R614.学号：TS100012贵阳医学院2013届硕士学位论文异丙酚预处理对大鼠两种急性呼吸窘迫综合征模型的保护作用比较研究生：程玉梅导师：沈锋教授年级：2010级专业：麻醉学2013年04月17日1贵阳医学院2013届硕士研究生论文目录摘要„„„„„„„„„„„„„„„„„„„„„„„„3前言„„„„„„„„„„„„„„„„„„„„„„„„6材料与方法„„„„„„„„„„„„„„„„„„„„„„8结果„„„„„„„„„

2、„„„„„„„„„„„„„„„13讨论„„„„„„„„„„„„„„„„„„„„„„„„18结论„„„„„„„„„„„„„„„„„„„„„„„„22参考文献„„„„„„„„„„„„„„„„„„„„„„„23英文摘要„„„„„„„„„„„„„„„„„„„„„„„26致谢„„„„„„„„„„„„„„„„„„„„„„„„30略缩词表„„„„„„„„„„„„„„„„„„„„„„„31论文原创性声明„„„„„„„„„„„„„„„„„„„„32附：综述„„„„„„„„„„„„„„„„„„„„„„332贵阳医学院2013

3、届硕士研究生论文异丙酚预处理对大鼠两种急性呼吸窘迫综合征模型的保护作用比较麻醉学（重症医学）研究生：程玉梅导师：沈锋摘要目的比较异丙酚(propofol)预处理对大鼠盐酸（HCl）吸入性和油酸（OA）静脉注射诱导性急性呼吸窘迫综合征（ARDS）的保护作用。方法分别通过气管内滴注HCl（0.1mol/L，2ml/kg）及尾静脉注射OA（0.1ml/kg）复制大鼠ARDS模型。将56只成年健康雄性SD大鼠随机分为7组：对照组、HCl组、HCl+异丙酚组[按异丙酚注射时间不同再分为2个亚组：HCl吸入前24小时

4、腹腔注射异丙酚组（HT24b组）、HCl吸入前12小时腹腔注射异丙酚组（HT12b组）]、OA组、OA+异丙酚组[按异丙酚注射时间不同再分为2个亚组：OA注入前24小时腹腔注射异丙酚组（OT24b组）、OA注入前12小时腹腔注射异丙酚组（OT12b组）]。以HCl气道滴注后及OA注射后6小时为实验终点；测定大鼠动脉血氧分压（PaO2）并计算氧合指数，测定支气管肺泡灌洗液(BALF)总蛋白（TP）含量、肺组织湿/干重比（W/D），对BALF行有核细胞计数及分类计数，测定肺组织匀浆髓过氧化物酶（MPO）活性；

5、光镜下观察肺组织病理改变并行肺组织损伤程度评分。结果HCl组和OA组大鼠造模成功后迅速出现呼吸加快加深、发绀、活动减少，随后症状进行性加重；HT24b组与OT24b组分别较HCl组和OA组有所减轻；而HT12b组与OT12b组症状较HCl组和OA组无明显改善。HCl组及OA组光镜下见不同程度的肺泡腔及肺间质水肿、出血，间隔增宽，中性粒细胞渗出，毛细血管充血等病理改变；HC1组主要表现为肺组织结构的毁损，肺泡腔内大量的水肿液、纤维蛋白及中性粒细胞渗出，肺内皮细胞坏死脱落，而肺间质水肿、间质内炎细胞浸润相对较

6、轻；OA组则主要表现为肺泡间隔的水肿、出血，大量炎症细胞聚集在肺间质中，肺泡毛细血管内充血，而肺组织结构的毁损，肺泡腔内水肿、炎细胞浸润相对较轻。HT24b组及OT24b组肺组织病理改变较HC1组、OA组明显减轻，而HT12b组、OT12b组与HCl组、OA组比较病理改变严重程度相似。与对照组比较，HCl组、OA组大鼠氧合指数均显著降低[（248±37）mmHgvs.(388±23)mmHg；(292±33)mmHgvs.(388±23)mmHg，均P=0.000]；肺组织损伤程度评分显著升高(10.90

7、±1.38vs.1.50±0.14；10.00±1.17vs.1.50±0.14，均P=0.000)；BALF中TP含量显著升高[（0.94±0.14）mg/mlvs.(0.28±0.09)mg/ml，(0.77±0.08)3贵阳医学院2013届硕士研究生论文mg/mlvs.(0.28±0.09)mg/ml，均P=0.000]；W/D显著增加(5.03±0.60vs.3.92±0.28；4.83±0.36vs.3.92±0.28，均P=0.000);BALF中有核细胞数[(2.393999±0.828)×

8、10/Lvs.(0.037±0.028)×10/L；(1.166±0.499)×10/L9vs.(0.037±0.028)×10/L，均P=0.000]、中性粒细胞百分比[(22.30±4.41)%vs.(5.40±0.51)%；（17.70±2.96）%vs.（5.40±0.51）%，均P=0.000]显著增加；肺组织匀浆MPO活性显著增加[(1.55±0.15)单位/克湿片vs.(0.35±0.06)单位/克湿片；(1.4