中药补肾与清热组分对小鼠肺癌伴忧郁模型的影响

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1、後里大学万方数据硕士学位论文(科学学位)中药补肾与清热组分对小鼠肺癌伴忧郁模型的影响院系：专业：姓名：指导教师：导师组成员：华山医院中西医结合临床吴晓董竞成教授吴金峰、段晓虹万方数据复旦大学硕士研究生学位论文目录主要缩略词表⋯·······⋯·················．·．．···．⋯⋯⋯⋯1中文摘要·················⋯···⋯·········-················2英文摘要·⋯⋯···⋯·⋯······················．．．．··．⋯．．．3前言···················⋯···⋯···⋯⋯

2、··⋯．···．．．···4正文第一部分社交应激性抑郁模型的建立及其效果评价···⋯··········9材料与方法··········⋯··························⋯·⋯⋯···9结果··················⋯-····⋯·················⋯··13讨论⋯⋯····⋯⋯⋯································16第二部分不同时段的社交应激对小鼠肺癌生长及其转移的影响······18材料与方法·⋯⋯·····⋯⋯⋯······························18结果

3、················⋯⋯⋯⋯···⋯·····⋯⋯·⋯··22讨{仑⋯·······⋯··⋯⋯⋯···························27第三部分补肾组分淫羊藿苷及淫羊藿素对小鼠社交应激性抑郁的干预影响·⋯·⋯⋯⋯··········⋯⋯⋯⋯·-·⋯·······⋯·-·⋯·3l材料与方法··················································3l结果··⋯····⋯···⋯⋯⋯⋯⋯·····⋯⋯⋯·⋯··33讨{仑····································

4、··············36第四部分补肾组分淫羊藿苷及清热组分汉黄芩素对小鼠社交应激性抑郁伴肿瘤的干预研究···········································39材料与方法···············⋯···⋯······⋯··⋯··⋯一⋯一39结果····⋯·········································一40讨{仑····⋯···⋯··⋯⋯⋯⋯···⋯··⋯⋯·⋯⋯··46全文总结··················⋯⋯⋯·一⋯··．．····一一⋯一48参考文献····⋯···⋯

5、··⋯⋯⋯⋯···⋯··一····一一···一51综述·········“·······················-··⋯·⋯·⋯··62致i射⋯⋯···⋯···⋯⋯⋯⋯··一··一····”·”······72万方数据复旦大学硕士研究生学位论文主要缩略词表DMEMDulbecco’SModified达尔伯克改良伊格尔培Eagle’SMedium养基FBSFetalbovineserum胎牛血清PBSPhosphatebuffer磷酸盐缓冲液solutionBDNFBrainderived苯甲基黄酰氟化物neurophicfactorVEGFVascu

6、larendothelial血管内皮生长因子growthfactorL，CAML1celladhesionL1细胞粘附分子moleculeIL-6Interleukin-6白细胞介素一6SDSSodiumdodecylsulfate十二烷基硫酸钠TBSTTriS-bufferedsalineTris缓冲盐及吐温-20Tween-20MMP-2Matrix基质金属蛋白酶2metalloproteinases2MMP-9Matrix基质金属蛋白酶9metal10proteinases9P-TrkBPhosphorylated磷酸化神经营养素受体tyrosinek

7、inasereceptorBP-ErkPhosphorylated磷酸化细胞外信号调节extracellular激酶regulatedkinase1万方数据复旦大学硕士研究生学位论文中文摘要目的以小鼠皮下肿瘤的生长及其转移等为主要的观察指标，评价补肾组分淫羊藿苷及清热组分汉黄芩素对肺癌伴抑郁模型的干预效应。从肿瘤生长及转移相关因子和炎症因子的表达等环节，探讨补肾组分淫羊藿营及清热组分汉黄芩素抗肿瘤的作用机制。方法1、建立社交应激性抑郁模型，用Noldus行为学监测系统，监测小鼠行为学的变化。2、建立不同时段的肺癌伴社交应激性抑郁模型，用Noldus系统监测小

8、鼠的行为学，运用Elisa方法检测小鼠血清中VEGF