鼠源aafp蛋白对ige介导小鼠过敏反应的干预作用及机制研究

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1、《中国图书资料分类法》单位代码：10660分类号：R94学号：S110081贵阳医学院2014届硕士学位论文鼠源AAFP蛋白对IgE介导小鼠过敏反应的干预作用及机制研究研究生：王静娴导师：兰燕宇教授王爱民教授年级：2011级专业：药剂学2014年5月20日万方数据目录摘要.........................................................................................................1前言............................

2、.............................................................................41.IgE介导过敏性疾病的流行状况....................................................42.IgE介导过敏性疾病的发病机理....................................................43.IgE介导过敏性疾病的药物治疗手段...................................

3、........64.FcRIIb受体在调节IgE介导过敏反应中的作用........................65.立题依据.........................................................................................66.创新点..............................................................................................7第一部分融合蛋白mAAFP的构

4、建及表达...........................................81.1实验材料.....................................................................................101.2实验方法.....................................................................................121.2.1基因的克隆..........................

5、...............................................121.2.2插入载体pcDNA5.............................................................121.2.3大肠杆菌Top10感受态细胞制备...................................121.2.4连接产物电转化Top10感受态细胞...............................131.2.5挑选正确的亚克隆...................

6、.........................................13TM1.2.6重组质粒pcDNA5-mAAFP转染Flp-InCHO细胞....13TM1.2.7Westernblot检测蛋白在Flp-InCHO细胞的表达.......141.2.8亲和纯化目的蛋白.............................................................141.2.9SDS-PAGE蛋白电泳检测mAAFP纯度..........................14万方数据1.2.10H

7、PLC法检测mAAFP纯度.............................................141.2.11Westernblot确证纯化蛋白..............................................151.3.结果.............................................................................................151.3.1pcDNA5-mAAFP重组质粒的鉴定................

8、...................151.3.2Westernblot检测蛋白的表达............................................161.3.3W