山奈酚对脑缺血再灌注大鼠的神经保护作用与其机制分析

《山奈酚对脑缺血再灌注大鼠的神经保护作用与其机制分析》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、重庆医科大学硕士研究生学位论文和尼莫地平组。结论山奈酚对局灶性脑缺血再灌注损伤具有神经保护作用功效，其机制可能是通过调节TLR4/MyD88/NF-κB通路的表达。关键词：山奈酚，脑缺血再灌注，超微结构，TLR4，MyD88，NF-κB4万方数据重庆医科大学硕士研究生学位论文NEUROPROTECTIVEEFFECTSOFKAEMPFEROLAGANSTFOCALCEREBRALISCHEMIAREPERFUSIONINRATSANDITSMECHANISMABSTRACTObjective:Toevaluatetheneuroprotect

2、iveeffectsanditsmechanismofkaempferolonfocalcerebralischemiareperfusion(I/R)inrats.ToobservetheeffectofkaempferolonhippocampalneuronsultrastructureandTLR4(Toll-likereceptor4,TLR4)/MyD88(myeloiddifferentiationfactor88,MyD88)/NF-κB(nuclearfactor-κB,NF-κB)pathwayafterfocalcere

3、bralischemiareperfusion(I/R)inrats.Methods:1.Groupingofexperimentalanimals:OnehundredandtwentymaleSDrats,weighing260～300g,wererandomlydividedintosixgroups:-1-1shamoperationgroup,MCAOgroup,low(5mg·kg),middle(10mg·kg)-1andhigh(20mg·kg)dosesofkaempferolgroupsandnimodipinegroup

4、.2.Focalcerebralischemiainratswasestablishedbyinsertingamonofilamentsutureintointernalcarotidarteryfor1.5handthenreperfusedfor24h(MCAO).Theeffectsofkaempferolonneurologicaldeficitscores,theinfarctionvolumeofbrainandthepathologicalchanges5万方数据重庆医科大学硕士研究生学位论文afterHEstainwereo

5、bserved.Andthechangesofhippocampalneuronsultrastructureineverygroup’sratswereobservedbytransmissionelectronmicroscopy.3.TheexpressionofTLR4,MyD88andNF-κBp65incortexandhippocampalneuronsofratswasdeterminedbyimmunohistochemistry.AndthecontentsofTNF-αandIL-1βinserumweremeasure

6、dbyELISAkits.Results:1.Theneurologicalscores:theshamoperationgrouphadnoneurologicalsymptomsdefect.TheneurologicalscoresofMCAOgroupwerehigherthanthatofshamoperationgroup(p<0.01),whichprovedthismodelwassuccessful.ComparedwiththeMCAOgroup,kaempferol10-1-1mg·kg,20mg·kgadministr

7、ationgroupsandthenimodipinegroupcansignificantlyreduceneurologicalscores(p<0.01).Andtherewasno-1significantdifferencebetweenkaempferol5mg·kgadministrationgroupandtheMCAOgroup(p>0.05).2.Infarctionvolume:theshamoperationgrouphadnowhiteinfarctionvolume.Butcomparedwiththeshamop

8、erationgroup,theMCAOgroupshowedsignificantinfarctionvolume(p<0.01),whichprovedthis