皂角提取物对急性脑缺血再灌注大鼠的保护作用与其机制分析

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1、四川人学顾上研究生论文皂角提取物对急性脑缺血再瑾注丈鼠的保护作用及其机制研究ProtectiveEffectandMechanismStudyoftheextractiveofGleditsiasinensisLam(GSL)totheRatsduringAcuteCerebralIschemiaReperfnsionInjuryM叠jor：pharmacologyPostgraduate：FanKehuaTutor：YangFangjuprofessorAbstractOBJECTIVE：Intheexperiment,weestablishedthemodelofacutecerebral

2、ischemiareperfusionOat)inratstoobservetheeffectsonnfrvesfunctiondamages、cerebralinfarctionvolume、malondialdehyde(MDA)contentin翻x姗、myeloperoxidase(mo)andnitricoxide(No)contentinbraintissue、tulnornecrosisfactor-[alpha](TNF·Ⅱ)andnuclearfactorKappaBO砸·xB)expressedinbraimaf【cradminimefingthedifferentdosa

3、gesofextractiveofGleditsiasinensisLain(GSL)bymouth．WeestimatedthepossibleprotectmechanismofextractiveofGSLtotheratsduringcerebralischemiareperfusioninjury,andthepossibilityofGSLtoelitecerebralischemiadi8easeinclinic．METHODS：WeestablishedthemodelofacutefocalcerebralI／Rbymiddlecerebralarteryocclusion(

4、MCAO)withclew．72SDmaleratswererandomlydividedinto6groups：sham-operationgroup,cerebralI／Rmodelgroup(negativecontr01)，complexSalviaMiltiorrhizagroup(positivecontrol，32mg／kg’d．1)，extractiveofGSLgroups(150mg／kg·d-1，75mg／kg·d-1and38mg／kg·d．1)．Medicationgroupswfresuccessiveadministered3daysbeforeoperation

5、,andthenbuiltthecerebralI／Rmodels，howeverratsinsham-operationgroupWfreonlysewuptheskinafterexposingthecommoncarotidartery(CCA)、externalcarotidartery(ECA)、4四川大学硕}研究生论文皂角提取物对急性脑缺血再灌注人鼠的保护作用及其机制研究internalcarotidartery(ICA)intherightside．After2hoursofMCAO，wepulledouttheclewtorecoverythecerebralbloodsupp

6、ly．Andafter24hoursofreperfusion,neurologicaldeficitwercscored,cerebralhistomorphologywasobservedbytightmicroscope；andthecerebralinfarctionvolumewaSmeaSuredby2，3，5·triphenytetrazoliumchloride(TTC)stainingtechnique；NO、MPO、MDA、1NF—a、NF．KBlevelswel"edetectedbychemicalandimmBno-hlstochemicalmethods．RESUL

7、TS：WeestablishedcerebralI／RmodelsuccessfullyinratsbyMACO，andtheratsinmodelgroupappearedobviouslyethologychangesafter24hoursofcerebralI／R,thencerebralhistomorphologychanged,Noproductionincreased．inflam