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ID:33987471
大小:4.59 MB
页数:69页
时间:2019-03-03
《体外冲击波结合自体骨髓间充质干细胞移植治疗骨不连的实验分析》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、中文摘要VEGF阳性表达都强于对照组13.45±3.21(P<0.01),A组VEGF阳性数分别为52.18±5.63,48.27±3.35均好于B、C组(P2、时则较6周时明显减弱,对照组在各时相点无荧光标记。各实验组MAR在2周时最大,分别为:4.53±0.12,3.11±O.08,3.64±0.17(um/d),显示结果与VEGF免疫组织化学染色相吻合。结论:ESWT结合自体BMSCs移植治疗骨不连缩短了治疗时间,提高了骨缺损处VEGF的表达和骨痂量的生成,更好的促进了骨折的愈合,优于ESWT治疗和自体BMSCs移植治疗。关键词:体外冲击波疗法;骨髓间充质干细胞;自体移植;骨不连英文摘要TheexperimentalStudyofNonunionTreatmentwithExtracorporea3、lShockWaveTherapyandAutograftofBoneMesenchymalStemCellsABSTRACTobjective:Approximately5.10%ofallbonefracturesarecomplicatedbythedevelopmentofanonunion,whichiSdifficulttocureindepartmentoforthopedics.Inprimary1990,ExtracorporealShockWavetherapy(ESWT)wasusedsuccessfullyinfunda4、mentalandclinicalresearchwithoutcombiningstemcells,whichleadingtomanypositiveachievements.Inrecentyears,stemcelltransplantationandtissueengineeringinrepairingbonedefectsisahotspotsofglobalstudy.Bonemesenchymalstemcells(BMSCs)possessingofself-replication,self-renewal,multi.di5、fferentiationandthepotentialtobecomeosteoblastchondrocytesiSanidealseedcellinbonetissueengineering.ThisstudywillapplyESWTandautograftofBMSCsandusesimpleESWTorBMSCstransplantationtocurerabbitssufferedfrombonenonuion,observethreedifferentapproachstoinfluenceofbonenonuionhealin6、g.Theresearchwillexploreanoriginaltreatmentmethodtocurebonenonunionandprovidesomebenefitsforclinic.Methods:39NewZealandrabbitswereconfirmedbyradiographytohaveanonunioninbothradiusafter10weeksofrabbitsmodelestablished.AUanimalsweredividedintofour5、英文摘要groupsrandomally,whichGr7、oupAwastreatedbyESWTcombiningwithautograftofBMSCs.GroupBbysimpleautograftofBMSCs,GroupCbyESWTandGroupDbynothing.Underasepticconditions,BMSCswerecollectedfromthemedialfemoralofGroupAandB’Sanimals.ShockwavewasappliedinGroupAandCwhileGroupAandBreceivedautograftofBMSCstotreatbon8、enonunion.Beforetreatmentandat2,6,and10weeksaftertreatment,radiographicfoll
2、时则较6周时明显减弱,对照组在各时相点无荧光标记。各实验组MAR在2周时最大,分别为:4.53±0.12,3.11±O.08,3.64±0.17(um/d),显示结果与VEGF免疫组织化学染色相吻合。结论:ESWT结合自体BMSCs移植治疗骨不连缩短了治疗时间,提高了骨缺损处VEGF的表达和骨痂量的生成,更好的促进了骨折的愈合,优于ESWT治疗和自体BMSCs移植治疗。关键词:体外冲击波疗法;骨髓间充质干细胞;自体移植;骨不连英文摘要TheexperimentalStudyofNonunionTreatmentwithExtracorporea
3、lShockWaveTherapyandAutograftofBoneMesenchymalStemCellsABSTRACTobjective:Approximately5.10%ofallbonefracturesarecomplicatedbythedevelopmentofanonunion,whichiSdifficulttocureindepartmentoforthopedics.Inprimary1990,ExtracorporealShockWavetherapy(ESWT)wasusedsuccessfullyinfunda
4、mentalandclinicalresearchwithoutcombiningstemcells,whichleadingtomanypositiveachievements.Inrecentyears,stemcelltransplantationandtissueengineeringinrepairingbonedefectsisahotspotsofglobalstudy.Bonemesenchymalstemcells(BMSCs)possessingofself-replication,self-renewal,multi.di
5、fferentiationandthepotentialtobecomeosteoblastchondrocytesiSanidealseedcellinbonetissueengineering.ThisstudywillapplyESWTandautograftofBMSCsandusesimpleESWTorBMSCstransplantationtocurerabbitssufferedfrombonenonuion,observethreedifferentapproachstoinfluenceofbonenonuionhealin
6、g.Theresearchwillexploreanoriginaltreatmentmethodtocurebonenonunionandprovidesomebenefitsforclinic.Methods:39NewZealandrabbitswereconfirmedbyradiographytohaveanonunioninbothradiusafter10weeksofrabbitsmodelestablished.AUanimalsweredividedintofour5、英文摘要groupsrandomally,whichGr
7、oupAwastreatedbyESWTcombiningwithautograftofBMSCs.GroupBbysimpleautograftofBMSCs,GroupCbyESWTandGroupDbynothing.Underasepticconditions,BMSCswerecollectedfromthemedialfemoralofGroupAandB’Sanimals.ShockwavewasappliedinGroupAandCwhileGroupAandBreceivedautograftofBMSCstotreatbon
8、enonunion.Beforetreatmentandat2,6,and10weeksaftertreatment,radiographicfoll
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