体外冲击波结合自体骨髓间充质干细胞移植治疗骨不连的实验分析

《体外冲击波结合自体骨髓间充质干细胞移植治疗骨不连的实验分析》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、中文摘要VEGF阳性表达都强于对照组13．45±3．21(P<0．01)，A组VEGF阳性数分别为52．18±5．63，48．27±3．35均好于B、C组(P

2、时则较6周时明显减弱，对照组在各时相点无荧光标记。各实验组MAR在2周时最大，分别为：4．53±0．12，3．11±O．08，3．64±0．17(um／d)，显示结果与VEGF免疫组织化学染色相吻合。结论：ESWT结合自体BMSCs移植治疗骨不连缩短了治疗时间，提高了骨缺损处VEGF的表达和骨痂量的生成，更好的促进了骨折的愈合，优于ESWT治疗和自体BMSCs移植治疗。关键词：体外冲击波疗法；骨髓间充质干细胞；自体移植；骨不连英文摘要TheexperimentalStudyofNonunionTreatmentwithExtracorporea

3、lShockWaveTherapyandAutograftofBoneMesenchymalStemCellsABSTRACTobjective：Approximately5．10％ofallbonefracturesarecomplicatedbythedevelopmentofanonunion，whichiSdifficulttocureindepartmentoforthopedics．Inprimary1990，ExtracorporealShockWavetherapy(ESWT)wasusedsuccessfullyinfunda

4、mentalandclinicalresearchwithoutcombiningstemcells，whichleadingtomanypositiveachievements．Inrecentyears，stemcelltransplantationandtissueengineeringinrepairingbonedefectsisahotspotsofglobalstudy．Bonemesenchymalstemcells(BMSCs)possessingofself-replication，self-renewal，multi．di

5、fferentiationandthepotentialtobecomeosteoblastchondrocytesiSanidealseedcellinbonetissueengineering．ThisstudywillapplyESWTandautograftofBMSCsandusesimpleESWTorBMSCstransplantationtocurerabbitssufferedfrombonenonuion，observethreedifferentapproachstoinfluenceofbonenonuionhealin

6、g．Theresearchwillexploreanoriginaltreatmentmethodtocurebonenonunionandprovidesomebenefitsforclinic．Methods：39NewZealandrabbitswereconfirmedbyradiographytohaveanonunioninbothradiusafter10weeksofrabbitsmodelestablished．AUanimalsweredividedintofour5、英文摘要groupsrandomally,whichGr

7、oupAwastreatedbyESWTcombiningwithautograftofBMSCs．GroupBbysimpleautograftofBMSCs，GroupCbyESWTandGroupDbynothing．Underasepticconditions，BMSCswerecollectedfromthemedialfemoralofGroupAandB’Sanimals．ShockwavewasappliedinGroupAandCwhileGroupAandBreceivedautograftofBMSCstotreatbon

8、enonunion．Beforetreatmentandat2,6，and10weeksaftertreatment，radiographicfoll