自体骨髓间充质干细胞经皮注射修复骨缺损的实验分析

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1、军医进修学院硕士学位论文AnExperimentalStudyonPercutaneousAutologousCulturedBoneMarrowMesenchymaiStemCellGraftingforBoneDefectAbstractObjectives：Toestablishareproduciblesystemforthecultureandosteogenicdifferentiationofrabbitbonemarrow-derivedmesenchymalstemcells(

2、BMSCS)invitro，andalsot0improvetheaspirationandcultivationofBMSCs．Todetermineinvivoosteoganicpotentialinsize-criticalbonedefectafterpercutaneousautologousgraftingofculture-expandedrabbitautologousBMSCScombinedwiththoseinducedbyosteoganicsupplement(0S)

3、．Methods：1．BMSCSwereculturedandtheninducedwithOSmediummeanwhiletheywereobserveddailyandcellnumberwereanalyzed．ALPenzymeactivitywasalsomeasuredSOastofindouttheexactphasewhenMSCwouldhavethemaxiumosteogenicpotential．ThecellnumberandALPenzymeactivitywere

4、comparedbeforeandafterosteogenicinduction。2．Rabbitsweredividedinto4groupsatrandom．myeloidpunctureneedleswereappliedinGroupA，B·Cduringbonemarrowaspiration，andcommon20mlinjectionsyringewithcircularcone-shapedpinheadwereappliedingroupD．IngroupA，B，C'D’cu

5、lturemediumWasDMEM、DMEF--12、a--MEM．DMEMrespectively．Thecellnumberaftercultivatgionfor10daysandcellgrowthCUI'V伪ofF2wereinvestigated．3．the15mmbonedefectexperimentalmodelatbothradiusofrabbitwe坤dividedinto5groupsatrandom．BMSCSwithandwithoutOSinductionwer

6、ecollectedandpercutaneouslyautologousinjectedrespectivelyintoradiusbonedefects．ThegraftinGroupAWas1X106BMSCs+IX106osteo-induced．4．军医进修学院硕士学位论文BMSCs，ingroupBwas2X106osteo-inducedBMSCs,and2X106BMSCsingroupC．5mgBMPcombinedwithfibrinsealantwasimplantedin

7、groupDand0．9％NaCIsolutioninE．Ostcogenesisatthedefectareawasobservedbyregularradiography,histologyandbiomechanics．Results：1．thereWasnodiffernenceoncellnumber,shape，growthcurveofBMSCsbetweenmyeloidpunctureneedlesandcommon20mi叫ectionsyringewithcircularc

8、one-shapedpinhead2．BMSCsshowmuchhighergrowthrateandgreaterostcogenicpotentialwhenculturedina--MEMmedium，incomparisonwithDMEM、DMEF--12．3．AlargequantityofcallusWasfoundinthe4groupswhiletheblankcontrolgroupshowlittlecallus4weeksaftercelltrasplantation．A