丁苯酞对血管性痴呆大鼠认知障碍与其海马ss、p38mapk表达的影响

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1、和HE染色，同时可以在光学显微镜下进行病理细胞学观测。然后从四组大鼠的病理切片上选取4个显微视野界面进行检测，拍照并且测定病理图片的灰度数值，以取其的平均数值来作为该切片的最终病理结果，最后将所有的切片结果统一取个平均数值，通常采用均数士标准差(xs)进行数字表示。最后进行统计学的处理，P<0.05才具有统计学的意义。结果：四组实验动物在术后都表现出全身的毛发混乱、肢体活动变得较少、精神状态萎靡不振以及饮水、食欲不强。四组大鼠记忆能力强弱次序与学习能力强弱一样。假手术组中的最强，丁苯酞组次之，模型组最差。

2、四组大鼠之间学习、记忆能力分别用统计学处理，均有统计学意义，P<0.05。丁苯酞和奥拉西坦可减少大鼠的麻醉苏醒时间，在VaD大鼠模型发病的急性期，具有一定的促醒效果。结论：丁苯酞是通过降低SS和P38MAPK的表达以及阻断海马细胞凋亡来改善VaD大鼠在水迷宫试验中的学习和记忆能力；同时也能减轻VaD大鼠神经元水肿和变性，对慢性脑缺血损伤有一定的脑保护作用。关键词：血管性痴呆,丁苯酞，SS，P38MAPK，海马EffeetsofNBPontheintelligentdisorderandtheexpressi

3、onofSSaswellasP38MAPKinvaseulardementiaratsABSTRACTObjective:Vasculardementiaisageneraltermforaseriesoffactorsleadingtoadvancedcerebrovascularbraindysfunctioncausedbybraindamagesyndrome,acommonsequelaeofcerebrovasculardisease.Withthedevelopmentofsociety,pe

4、ople'slivingstandardsimprove,China'sagingpopulationisbecomingincreasinglyprominent.TheincidenceofVaDareconstantlyrising,hasbecomeacommonneurologicaldisease,notonlyseriouslyaffectthequalityoflifeofpatients,butalsotothefamily,thecommunityhasbroughtaheavyburd

5、en.Asananti-cerebralischemicdrugs,NBPhasbeenwidelyusedinthetreatmentofcerebrovasculardisease,takingtheNBPforthetreatmentofcerebralinfarctionissafeandeffective.ButthestudyoftherelevantButylphthalidetreatmentofstrokeVaDiscurrentlysmall.Pharmacologicalmechani

6、smTherefore,thestudyoflearningandmemoryButylphthalide,hippocampus,SS,andtheimpactofP38MAPKratswithvasculardementia,explorethepathogenesisofVaDandantiVaDdrugshaveveryimportanttheoreticalandpracticalsignificance.Methods:12-14month-oldmaleWistarratsweredivide

7、dintoshamoperationgroup,modelgroup,oxiracetamgroupButylphthalidegroups.Inthisstudy,bothsidesofthecommoncarotidarteryligationmethodtoproduceananimalmodelofVaD.Wecanbethreegroupsofrats(modelgroup,groupandButylphthalideoxiracetamgroup)wereusingalongthesideoft

8、hecommoncarotidsurgicalligationoftwolines,andthenstartfromthemiddlepartofthecut,andthensuturedincisionintheskin.Postoperativewarm,tobewakingup,cleaningcagesyardputreturntocontinuefeeding.Inadditiontotheshamgr