sirna介导的clc-3基因沉默对h2o2诱导的pc-12细胞凋亡的影响

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1、中图法分类号：R33学校代码：10661学号：2011410密级：公开硕士学位论文（学术型学位）siRNA介导的ClC-3基因沉默对H2O2诱导的PC-12细胞凋亡的影响EffectsofsiRNA-mediatedClC-3genesilenceonhydrogenperoxideinducedPC-12cellapoptosisinculture姓名：李曦专业：生理学研究方向：脑缺血的保护机制导师：王春梅教授培养单位：遵义医学院2014年5月万方数据李曦遵义医学院硕士学位论文万方数据李曦遵义医学院硕士学位论文目录1．论文：

2、siRNA介导的ClC-3基因沉默对H2O2诱导的PC-12细胞凋亡的影响中英缩略词对照表„„„„„„„„„„„„„„„„„1中文摘要„„„„„„„„„„„„„„„„„„„„„3英文摘要„„„„„„„„„„„„„„„„„„„„„5前言„„„„„„„„„„„„„„„„„„„„„„„7材料与方法„„„„„„„„„„„„„„„„„„„„9结果„„„„„„„„„„„„„„„„„„„„„„„19讨论„„„„„„„„„„„„„„„„„„„„„„„28结论„„„„„„„„„„„„„„„„„„„„„„„31参考文献„„„„„„„„„„„„„„

3、„„„„„„„322．综述：微小RNA与脑卒中发病机制研究„„„„„„„353．致谢„„„„„„„„„„„„„„„„„„„„„„„454．作者简介„„„„„„„„„„„„„„„„„„„„„46基金课题编号：国家自然科学基金（81160157）和贵州省科技厅基金（20093075）万方数据李曦遵义医学院硕士学位论文中英文缩略词表缩略词英文全称中文全称AVDapoptoticvolumedecrease细胞凋亡性容积减少BCAbicinchoninicacid2,2-联喹啉-4,4-二甲酸二钠Caspasecysteine-con

4、tainingaspartate-半胱氨酸天门冬氨酸蛋白酶specificproteasesClCvoltage-dependentClchannels氯通道CCK-8Cellcountingkit-8细胞计数试剂盒ddH2ODoubledistilledwater双蒸水DMEMDulbecco`sMinimumEagleDMEM培养基MediumDMSODimethylsulphoxide二甲基亚砜dsRNADoublestrandedRNA双链RNAEDTAEthylenediamineteraaceticacid乙二胺四

5、乙酸FBSFetalbovineerum胎牛血清GAPDHglyceraldehyde-3-phosphate甘油醛-3-磷酸脱氢酶dehydrogenaseH2O2hydrogenperoxide过氧化氢HRPhorseradishperoxidase辣根过氧化物酶KDaKilodalton千道尔顿miRNAMicroribonucleicacid微小核糖核酸mRNAmessengerribonucleicacid信使型核糖核酸ntnucleotide核苷酸ORCCoutwardrectifyingchloride外向整流氯

6、离子通道channelPAGEPolyacrylamidegel聚丙烯酰胺凝胶电泳electrophoresis1万方数据李曦遵义医学院硕士学位论文PBSPhosphatebufferedsaline磷酸盐缓冲液PC-12Pheochromocytoma大鼠肾上腺嗜铬细胞瘤细胞PCDprogrammedcelldeath程序性细胞死亡PTGSPost-transcriptionagene转入后基因沉默silencingReal-TimeReal-Timerevers实时定量逆转入聚合酶链性反RT-PCRtranscriptio

7、n-polymerasechain应reactionPLLpoly-L-Lysine多聚左旋赖氨酸PMSFphenylmethanesulfonylfluoride苯甲基磺酰氟RNAiRNAinterferenceRNA干扰RISCRNA-inducesilencingcomplexRNA诱导沉默复合物siRNAsmallinterferingRNA小干扰RNASDSsodiumdodecylsulfate十二烷基硫酸钠SDS-PAGESDS-polyacylamidegelSDS-聚丙烯凝胶电泳electrophoresos

8、SuperECLsuperECLplusdetection超敏感发光液PlusreagentTBSTrisbuffersolutionTris缓冲盐溶液VRACvolume-regulatedanionchannels容量调节阴离子通道2万方数据李曦遵义医学院硕士学位论文si