丁苯酞预处理对脑缺血再灌注大鼠皮质神经元超微结构和白介素-1β的影响-(5394)

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1、中文摘要5、各组白介素-1β含量数值比较与Sham组比较，余各组大脑皮质白介素-1β含量均明显增高（P<0.01），NBP低剂量组白介素-1β含量较IR组减少（P<0.05），NBP中、高剂量组白介素-1β含量较IR组减少（P<0.01），有显著意义。NBP高剂量组与中、低剂量组两两比较白介素-1β含量均有显著性差异（（P<0.01）。结论：1、NBP预处理组的大鼠大脑皮质梗死区，较Sham组及IR组均明显缩小。提示丁苯酞预处理可减小脑缺血后脑梗死体积。2、NBP预处理组较IR组神经功能缺损评分明显减少，光镜及电镜下可显示大脑皮质神经元破坏程度较小，保

2、留部分线粒体，神经元损害较轻。提示丁苯酞预处理对神经元有预防性保护作用。3、电镜下提示，在Sham组未见白细胞浸润，但IR组及NBP预处理组均明显增多，但NBP各组的炎性细胞浸润较IR组明显减少；白介素-1β在NBP预处理组较IR组含量明显较少，提示丁苯酞可能通过抑制白介素-1β的过度表达，减小炎性反应来参与神经元的预防性保护作用。4、NBP各剂量组比较：高剂量组较中、低剂量组梗死体积较小，神经元结构损伤较轻，炎性细胞浸润较少等提示高剂量组较低剂量组预防更有效。关键词：缺血再灌注；丁苯酞；白介素-1β；预处理；超微病理技术3英文摘要Theeffecto

3、fbutylphthalidepreconditioningoncorticalneuronultrastructureandinterleukin1betainratsaftercerebralischemiareperfusionABSTRACTIschemiccerebrovasculardiseaseisassociatedwithhighmorbidity,highdisabilityandhighmortality,andsignificantlyimpactthehealthofhumanbeing.Neuronsareunusually

4、sensitivetoischemia.Thecerebralischemiaresultsinnecrosisandapoptosisofnervecells，andcancauseseriousandevenirreversibleneurologicdeficit.Therefore,itisimportanttostimulatetheendogenousprotectivemechanismforimprovingresistanceofnervecelltoischemia，lesseningthenervousdamageafterisc

5、hemiareperfusion,andmaintainthenormalphysiologicalfunction.Researchhasshownthatsomesubstancesindicateantagonizingeffectsonnervecellsinexperimentalcerebralischemia，suchasadenosineandheatshockprotein(HSP).Racemic-3-butylphthalide(NBP)isaclassofchemicaldrugsfortreatmentofacuteische

6、micstroke.NBPmayalleviatethenervecelldamageandimprovefunctionalrecovery.ResearchershaveprovedthatNBPhasprotectiveeffectsonthebrain.However，whetherthepretreatmentofNBPcouldimprovethetolerabilityofnervecellsisstillunclear.TheneuroprotectiveroleofNBPmayoccurthroughoneofthesemechani

7、sms:releaseofcytochromeCinthechannelofischemicneuronalapoptosis，protein-Smac/DLABLO、Caspasefamily,andHSP-70releasedbymitochondria，etc.Theinflammatoryreactionisthemajormechanismofcentralnervoussystemdisease.Abnormalactivationofgliosismayparticipateinthepathologicalcoursesofbraind

8、amage.Interleukin-1（IL-1）mainlyincludesIL-1αand