p2x,3嘌呤受体在三叉神经节伤害性感受神经元的表达及其作用

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1、华中科技大学同济医学院硕士学位论文P2X<,3>嘌呤受体在三叉神经节伤害性感受神经元的表达及其作用姓名：刘春明申请学位级别：硕士专业：神经生物学指导教师：李之望;李超英2002.4.1查苎：丝墨堡垡垄三墨丝篓芏丝堇壁兰丝丝重』丝垄垄塞塑一一AbstractThereisevidenceshowingthat：P2X3purinoceptorsubtypeisselectivelyexpressedinprimarysensoryganglionneurons，suchasinneuronsofratdorsalroot、trigeminalandnodosegangl

2、ia,anditismainlyexpressedinneuronsrangingfromsmalltomediumdiameter．Strongevidenceshowsthat，P2X3purinoceptorsubtypeiscloselyrelatedtothenocieeptionofprimarysensoryneurons．Inthepresentstudy,threegroupsofrattrigeminalganglionneuronsareclassifiedinaccordingtotheirdiameters：small(<30pro)(52．

3、62．85％，583／1108)、medium(30肛m-40ttm)(35．84％，397／1108)andlarge(>40pm)neurons(11．54％，128／1108)．IthasbeenobservedinthisexperimentusingwholecellpatchclamptechniquethatextracelinlarlyappliedATPeauinducecurrentsintherattrigeminalgan甜ionneurons，mostofwhichareinward．currents(53．6％，15／28)andother

4、sareoutward．(7．1％．2t28)orbiphasic—currents(3．6％，1／28)．ThereisacertaincorrelationbetweenthediameteroftheneuronsandthetwokindsofdesensitizationforATP-inducedinward-currents：smalldiameterneuronshaverapid-desensitizatingcurrents，andmediumdiameterneuronshaveslow—desensitizatingcurrents．ItPag

5、elof64丝苎：丝垄堡垡垄三墨丝茎丝堇壁堡丝丝篓垂兰丝垂垄堡兰塑——hasbeenidentifiedusingRT-PCRthatthereisexpressionofP2X3purinoceptorsubtypeinthetrigeminalganglionneurons·Furthermore，ithasbeenprovedbyinsituhybridizationthatP2X3purinoceptormRNAismainlyexpressedinsmalltomediumdiameterneurons．Inthetrigeminalgangliaslice

6、s，nociceptiveneuronsinnervatingdentalpulp，whicharelabelledbymeansofHRP．retrogradedneurotracing，arereferredtothesubsetofsmalldiametertrigeminalganglionneurons，inwhichthereisP2X3purinoeeptormRNAexpressingtoo．ThisfindingsuggeststhatP2X3purinoceptorisselectivelyexpressedinsubgroupsofsmallto

7、mediumdiameterneuronsinthetrigeminalgangliaanditiscloselyrelatedtothenociceptionofprimarysensoryneuronsoriginatedfromdentalpulp．Keywords：rattrigeminalganglionneuron；wholecellpatchclamprecording；RT—PCR：insituhybridization；HRP-retrogradedneurotracing；ATP；P2X3purinoceptor；noeicept