脾切除大鼠免疫功能改变对骨折愈合影响的实验分析

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1、万方数据·英文论著摘要·SplenectomyratsimmunefunctionchangeexperimentalstudyoftheeffectsonfracturehealingObjectiveClinicalcasesalecomplextrauma，thefactorsinfluencingthefracturehealingismore，thisexperimentthroughtheestablishmentofanimalmodeloffracturecombinedsplenectomybysinglefactorobser

2、vationaftersplenectomyimmunefunctionchangeimpactOilfracturehealing．Method1．Animalgroups：healthy12weeksSpraguewrote-Dawley(SD)ratswereratiomlydividedinto5groups：groupA：fiveexperimentalSDratsfemurfi'acture+splenectomygroupB：controlgroupinSDrats1mysimplyfemoralfracturesingroupC：

3、experiInentalSDratsmaysimplysplenectomygroupD：e冲eriIllentalSDmtsfemurfiacture+5fakesplenectomygroupE：5experimentalSDratsonlyfalsefracturegroup2．Thefractureanimalmodel：thejigsawopenfemoralfracturemodelisestablished，gramintramedullalyneedlefixed3．Splenectomyanimalmodelestablish

4、ment：departmentaccordingtotherat’Sabdomensurgery,conventionallaparotomyseparationmethods．excisionofspleen,completelyclosedabdomenafterhemostasis．False舶eaftersplenectomygroupfindthespleenspleen,withsplenectomygroup．4．X-my：fractureafter1week，2weeks，3weeks，6weeks，4weeks，8weeks，1

5、0weeks，12goXlinepiece，callusgrayIPP6．0softwareanalysis5．Peripheralblood：experimentalanimalsdiebeforetakingablood，2days，7days万方数据postoperatively,2weeks，3weeks，4weeksofperipheralbbod，realtime。RTPCRdeterminationofTNFalpha，IL一6，BMPmRNAexpressionlevel．ResuIt1．Fracturecombinedsplen

6、ectomygroupandsimplefi'acture，fracturecombinedsplenectomygroupandfalsefracturecombinedsplenectomy,callusregionalcomparisonbetweengroups，P’'0．05，therearedifferences，statisticallysignificant；simplefractureandfracturemergefalsesplenectomygroupandcornpar西onbetweencallusareagroups

7、，P>0．05，110significantdifference，nostatisticalsignificance·2．SplenectomygroupofTNFalpha，IL一6mRNAexpressionofaroseslightlyaftersplenectomy,peakedalter7days，afteradownwardWend．NotsplenectomyinthecontroIgroup，theTNFalpha，IL一6mRNAexpressioncomparedwithpreoperativehas110obviouscha

8、nge．3．ThefracturemergemRNAexpressionofBMPiIlsplenectomygroupshewedat