失神经支配对大鼠胫骨骨折愈合影响的实验分析

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1、consecutivedaysafterintramuscularinjectionofpenicillin10000units／kgbodyweight，threetimes／day,andTopicalapplicationofpovidone-iodinedisinfectionofwoundsthenextday,topreventwoundinfection．The。experimentalanimalsatvarioustimepointsafter4,7，14，21，28dayswerekilled5，twoda

2、ysandthepreviousweekbeforetheexecution，andtetracyclinehydrochloride(30mg／kg)bysubcutaneousinjection．Observed：ExperimentalanimalswerekilledafterthelineofXraycameratoobservelaboratoryanimalswithbilateralchangesintibialbonehealing，measuringandcomparingthegrowthofcallus

3、；Experimentalanimalsweresacrificedbeforetheremovalofthekneeandanklejointsaroundtheattachmentofmusclesandjointcapsuletoretainacompletetibia，visualobservationofthegrowthofcallusandtexture．Fractureofthetibialstump，includingcallus，hematomaandperiosteumwereremoved、砘thlig

4、htmicroscopeexamination，cardedouthistologicalanalysis，observationofboneeellproliferation，differentiation，andbonecalcificationWasconductedbyfluorescencedetection，observationdeposition，andeachgroupofdataforstatisticalanalysisanddrawingconclusions．Results1、Imagingexami

5、nation：FilmXraydetectionintheevaluationofbonecallus，After4daysand1weeknosignificantdifferencebetweenthetwogroupsofcallus，havenosignificantcallusgrowth；ThetwogroupshaveemergedaftertWOweeksofhigh-densityshadowintheexperimentalgroupmorecallusvolume，density，comparedwith

6、thecontrolgroupispoor,anduneven；After3weeks，theremorecallusinexperimentalgroupascomparedtocontralateral，andlowdensityinagreatmess；alargenumberofexperimentalgroupafter4weeksofcallusgrowth，comparedwiththecontrolgroupwasprominentcorticalbone，bonemineraldensityuneven．2、

7、Histologicalexaminationunderlightmicroscope：Hematomaafterfourdaysthetwogroupsarethemain，onlyasmallamountintoafibroustissue，nosignificantdifferencebetweenthetwogroups；1weekaftersurgery,theexperimentalgroupalargenumberoffibroustissueproliferation，fibrosiscontrolgroupa

8、ctiveinmesenchymalcellsincreasedandvisiblepartofthecartilageceils．Thetwogroups1weekaftertheresultsofthehistologicalscore：0．64-0．42，1．14-O．