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ID:33017020
大小:3.82 MB
页数:50页
时间:2019-02-19
《鞘注神经生长因子促进大鼠胫骨骨折愈合的实验分析》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、硕士学位论文EffectsofintrathecaladministrationofnervegrowthfactoronthehealingoftibialshaftfractureintheratName:CaoLiangGuoSuperviseWangGangervlsor:anRABSTRACTBackgroundNowadays,谢tllthedevelopmentofmodernmedicine,thenutritionalinteractionbetweenneuronandnon-neurontissueshasbeenre
2、cognizedextensively.InclinicalInstitutionsapatientwhogotaTraumaticBrainInjury(TBI)accompaniedwithalong-bonefractureisverycommon.Inthesepatientstheprocessofbonehealingwerefrequentlypromotedwithalargequantityofcallus,andaheterotopicossificationsometimesasaresult.Howthecentra
3、lnervesdoescontrolthefracturehealingisuncertain.Thenervegrowthfactor(NGF)andneuropeptidedistributeextensivelyinkindsoftissues.ResultsinforeignresearchesdemonstratedthattheNGFfromendogenousorexogenouscallalsostimulatethereleaseofsensoryneuropeptidesinspinaldorsalrootganglia
4、(DRG),changingthebiologicalactivitiesindifferentsystem.WethereforepresumedthattheNGFandneuropeptidemaybecomposedageneralregulationpathwaybetweencentralnerveandnon·neuralsystem.‘‘Dosethismechanismcontributetofracturehealing?’’SeldomreportshasbeenfoundbynOW.objective:Toexami
5、nedtheeffectofintrathecaladministrationofNGFonhealingoftibialfractureinratsandanalyzetheexpressionofcalcitoningene-relatedpeptide(CGRP)andsubstanceP(SP).TofindtheregulationmechanismbetweencentralnerveandABSTRACTnon—neuralsystemandtodemonstratethehypothesisbasedontherelatio
6、nshipbetweenNGFandneuropeptide.Methods1.AnimalinexperimentForty-eightadultmaleSDratswererandomizedinto2equalgroups:theNGFtreatedgroupandcontrols.Ineachgroup,foursubgroupswe他establishedseparatelyaccordingtotheobservationspots(尹,14m,21髓,28mday)afteroperation.2.Experimentalmo
7、delAllofratsinthestudyhadreceivedanartificialfracture011midshaftinrighttibia.Ak-wirewasinusedtofixthefractureintramedully.Usingaspecialmicroinjector,2.5SmNGFwasperfusedthoughPEl0micro-pipeintotheintrathecalspaceattheLilevelofvertebraefor2weeks(1pg/day).EqualvolumeofsalineW
8、asinjectedtothecontrols.3.BonecallusevaluationAt7m,14m,21n,28mdayafterNGFinjection,theani
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