hif-2α、vegf在子宫颈鳞癌组织中表达的临床意义及相关性分析

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1、南昌大学硕士学位论文HIF--2α、VEGF在子宫颈鳞癌组织中表达的临床意义及相关性分析姓名：张丽霞申请学位级别：硕士专业：妇产科学指导教师：吴海根201206摘要目的：通过检测宫颈鳞癌组织及正常宫颈组织中HIF．2a、VEGF的表达情况，分析其表达的差异和相关性，探讨HIF．2a、VEGF的表达在子宫颈鳞癌的临床意义。方法：1．收集64例子宫颈鳞癌组织和22例正常宫颈组织，采用免疫组织化学法(Elivision法)检测各组织中HIF．2a、VEGF因子的表达；2．采用实时荧光定量PCR(qRT-PCR)检测子宫颈鳞癌组织及正常宫颈组织标本中HIF．2a、VEGF因子的mRNA表达情况。结果：

2、1．实时荧光定量PCR分析结果表明：HIF．2amRNA及VEGFmRNA表达较正常宫颈组织显著增加(P

3、RACTObjective：ToinvestigatetheexpressionofHIF．2aandVEGFinnomlalcervicaltissueandcervicalcarcinoma，analyzingdifferencesandcon．elationbet、Ⅳeenthem，andtoexploretheirclinicalsignificance．Methods：1·Specimenssurgicallyresectedfrom64casesofcervicalcarcinomaa11d22casesofcervicalbenigndiseasewerecollected．Th

4、eexpressionofHIF．2aandVEGFinthemwasdetectedbyimmunohistochemistry．2．RealTimePCRwasusedtodetecttheexpressionofHIF．2aandVEGFinnormalcervicaltissueandcervicalcarcinoma．Results：1．TheresultfromRealTimePCRshowedthattheexpressionofHIF一2仅mRNAandVEGFmRNAincervicalcarcinomawassignificantlyhigherthanthatinnorm

5、alcervicaltissues(P<0。01)．Therearesignificantdifferencesbetweennormalcervicaltissueandcervicalcarcinoma．2．TheresultofimmunohistochemistryshowedthattherearelittleexpressionofHIF·2旺andVEGFinnormalcervicaltissues，andcervicalcarcinomatissueshadasignificantlyincreasecomparedwiththatinnormalcervicaltissue

6、s(P<0．011．Andtherewasasignificantpositivecorrelationbetweenthetwofactors．Conelusion：1．ThehighexpressionofHIF一2aandVEGFincervicalcarcinomamayplaVanimpoaantroleduringtheinitiationanddevelopmentofcervicalcarcinoma．2．ThereWasasignificantpositivecorrelationbetweenHIF．2QandVEGFfactors．ThehighexpressionofH

7、IF-2QandVEGFincervicaltissuesmaybeinvolvedintheprogressofcervicalcarcinoma．KeyWords：CervicalCarcinoma；hypoxia—induciblefactor-2alpha(HIF．2Q)；vascularendothelialgrowthfactor(VEGF)中英文缩略词表英文缩写m剐NAHIF．10【