x线诱导鼻咽癌辐射抗拒细胞株的建立与机制初探

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1、中山大学硕士学位论文X线诱导鼻咽癌辐射抗拒细胞株的建立及机制初探Results1．TheradioresistantcelllineCNE2RofNPCisobtainedAccordingtotheresultsofMTT,thespeedofCNE2RisslowerthanCNE2，andthedoubletimeofCNE2Ris4．0dayswhilethatofCNE2is3．6days．Thedose—survivalcurveofCNE2RandCNE2shoWSthatthecurveofCNE2RiSflatterthanthatofCN

2、E2．TheparametersoflinearsecondarymodelofCNE2RandCNE2area(1．076vs0．974)，瞰一0．302vs一0．319)，SF2(O．5861vs0．3964)；．2．ThehereditarydiscrepancyofthecelllinesCNE2RandCNE2Accordingtothechromosomekaryotyping，thediscrepancyofcellularchromosomebetweenCNE2RandCNE2isobvious．STRanalysisshowsthatth

3、ereareprominentdiscrepancyoftheexpressionofSTRlocusD8S179inCNE2RandCNE2．3．ResearchesonthediscrepancyofthecelllinesCNE2RandCNE2Accordingtogenechip，thereare855genesexpresseddifferently．ComparetoCNE2，thereare538genesup—regulationand317genesdown-regulationinCNE2R．Thesegenesinvolvedinma

4、nycellularactivities，suchasapoptosis、binding、metabolism、cellcycle，signaltransduction，transcription，translation，structureandtransportofsubstancesetalInthiscurrentstudy,chromosome8and13arefoundthattheymaybeinvolveinthediscrepancyofradioresistancebetweenCNE2RandCNE2．2D—DIGEshowsthatth

5、ereare16spotsdifferentinCNE2RcomparedtoCNE2．Theproteins14—3—3zetaandCASP1maybeinvolveintheradioresistanceofNPC．Conclusions1．RadioresistantNPCcelllineCNE2Rhasbeenmadeupsuccessfully；．2．ComparetothecelllineCNE2，theradioresistanceofCNE2Renhanced．3．Thediscrepancyofradioresistancebetween

6、CNE2RandCNE2isrelatedwiththeirhereditarydiscrepancyonchromosome8and(0r)chromosome13．4．ThediscrepancyofradioresistancebetweenCNE2RandCNE2isrelatedwiththedifferentialexpressionofgenesandproteins，andbothproteins14—3-3zetaandCASP1maybeinvolveintheradioresistanceofNPC．KeyWords：Nasophary

7、ngeaiNeoplasms；RadiationTolerance；Karyotyping；Proteome；fluorescencetwo-dimensionaldifferencegelelectrophoresis—V一中山大学硕士学位论文X线诱导鼻咽癌辐射抗拒细胞株的建立及机制初探缩略词NPCVEGF主要英文缩略词英文全称NasopharyngealcarcinomaVascularendothelialgrowthfactormiRNAMicroRNAMTT3一(4，5一dimethylthiazolyl-2)一2，SFSF2ST飚SSRDIABV

8、A中文全称鼻咽癌血管内皮生长因子微小RNA5-dip