弓形虫sag1、mic3单基因与sag1-mic3融合基因真核表达载体的构建与鉴定

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1、山东大学硕士学位论文ConstructionandidentificationofrecombinanteukaryoticexpressionPlasmidcontainingSAGlmdMIC3fromeresslon1asmlA1anToxoplasmagondiiGraduatestudent：TianXiaodongAdvisor：Prof．Gu0inminMajor：PathogenicBiologyAbstractToxoplasmagondiiisanobligateintrac

2、ellularopportunisticprotozoa，responsibleforhumanandanimaltoxoplasmosis，itwasfirstdiscoveredbyNicolleandManceaux，Frenchscholarin1908．Thereare5stagesinitslifecycle，theyaretrophozoite，cyst，shizont，gametocyteandoocyst，and，inthattrophozoite，cyst，oocystare

3、duetotransmissionandpathogenesis．Mostinfectersareasymptomatic．however，inpregnantwomenandimunocompromisedpatientsinfectioncancauseseverecomplications．Surprisedly，toxoplasmosiscanchangeinfecters’character．ItwasreportedbyscholarthatToxoplasma-positiresu

4、bjectshave10werintelligencethanToxoplasma-negatiresubjects，statisticaldataalsoshowed：thereissomelinkbetweentoxoplasmosisandschizophrenia．LivestockaresusceptibletoToxoplasmagondii，toxoplasmosisisofgreateconomicimportanceworldwidebecauseitcausesabortio

5、ns，stillbirth，andneonatallossinalltypesoflivestock，Infectedlivestockareanimportantsourceofinfectionforhumans．Atpresent，vaccineshowsgreatpotentialsonthepreventionagainsttoxoplasmosis．Thedevastatingeffectoftoxoplasmosisinbothhumansanddomesticanimalsmak

6、esitoneofthediseasesofgreateconomicimportanceinmanypartsoftheworld．Therefore，avaccinewhichwillprovidesterileimunityagainstToxoplasmagondiiisurgentlyrequired．Toxoplasmagondii’Svaccinedevelopsfromdeadvaccinetoattenuationvaccine，fromdirectextractingsurf

7、aceantigentopurifyingunitpricesubunitvaccinebygeneticengineering，nowtocompoundpolyvalent3山东大学硕士学位论文vaccine．Tachyzoiteispathogenicstage，thesurfaceoftachyzoiteisthemainlyattachedtargetofhostimmunesystem，Amongthesesurfaceantigens，P30isoneofthemostpromis

8、ingvaccinecandidates，whichwasfocusedOilfrequently。P30isastage—specificantigen，onlyexpressedintachyzoitestage，covered3-5percentofthegrossproteinsofT．gondii，but，theantibodywhichitcaninducecover50percentoftotalantibody，serumantibodyrespondedtoP30weremea