右美托咪定不同处理方式对大鼠局灶性脑缺血再灌注损伤的干预分析

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1、万方数据四川医科大学硕士学位论文thisstudy．40ratsweredividedinto5groupsrandomly：theshamoperationgroup(Sham)；thecerebralischemic—reperfusiongroup(I／R)；thedexmedetomidinehydrochloridepreconditioninggroup(Dex1)；thedexmedetomidinehydrochtoridepost—conditioninggroup(Dex2)andthedexmedetomidinehydrochlorideprecon

2、ditioningunitedpost—conditioninggroup(Dex3)，8ratsineachgroup．Therightmiddlecerebralarteryocclusion-reperfusionmodelwasmadebyreformingLongamethod．30minutesbeforetheshamoperation，theshamgroupwasreceivedaninjectionofsaline5ml·kg"1byperitonealinjection，8hoursaftertheshamoperation，thenreceivedt

3、hesameinjectionamountofsaline．Allratswereobservedforanother19hours．30minutesbeforesurgery,bothI／RandDex2groupswerereceivedaninjectionofsaline5ml·kg-1byperitonealinjection．Thentherightmiddlecerebralarteryocclusion-reperfusionmodelWasmade．3hoursafterarteryocclusionand5hoursafterreperfusion，t

4、hentheI／Rgroupwasreceivedaninjectionofsaline5ml·k91byperitonealinjectionwhiletheDex2groupwastreatedwithDex50ug·kg-I．Eachgroupwasacceptedreperfusionfor24hours．30minutesbeforesurgery,bothDexlgroupandDex3groupswerereceivedaninjectionofDex50ug’k91byperitonealinjection．Thentherightmiddlecerebra

5、larteryocclusion—reperfusionmodelwasmade．3hoursafterarteryocclusionand5hoursafterreperfusion，thentheDex1groupwasreceivedaninjectionofsaline5ml·kg-1byperitonealinjectionwhile万方数据四JII医科大学硕士学位论文theDex3groupwastreatedwithDex50ug’kg-1．Eachgroupwasacceptedreperfusionfor24hours．27hoursafterreperf

6、usion，theneurologicaldeficitscoreswerecountedforeachgroup．Thebraininfarctvolumepercentagqof；bralinfarc"‘evaluatedbpercentageofcerebralintarctionsizewasevaluateoycomput‘erimageprocessingsystemafterstainedby2，3，5-triphenyltetrazolium—chloride(TTC)．Thesuperoxidedismutase(SOD)activityandmalona

7、ldehyde(MDA)contentinbraintissue，andtheneuronspecificenolase(NSE)contentinserumweremeasuredbyElisamethod．Result：1．EachgroupofSDrats’differencesinweightandagewerenotstatisticallysignificant(P>0．05)；2．ComparedwiththeShamgroup，intheI／Rgroup，Dexlgroup，Dex2groupand