wnt2fβ-catenin信号通路与骨关节炎发病机制的实验分析

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1、1．p天津医科大学硕士学位论文group．Thelevelsof13-cateninandMN田一13Weredetectedbyimmunohistochemistrystaining，andusingchi-squaretestorFisherexactprobabilityforstatisticanalysis．TotalRNAwasextractedfromarticularcartilagetissuesof0Apaitentsandcontrolgroup．RealTimereverset

2、ranscriptasepolymerasechainreaction(RT-PCR)WasperformedtotesttheexpressionofBMP·2，COL—XandMMP-13andalsotheexpresssionof夕eMWastestedforreference．nedatawasanalysedbySPSSstatistics7．0．Results1．ArticularcartilagedestructionswerefoundinOApatients，completeloss

3、ofarticularcartilageandchondrocytespremature，hypertrophy，cellcloning，ortidemarkbreakingandnewbloodvesselinvasionwerealsofoundinthesepatients．2．Theresultof13-cateninimmunohistochemistryshowednegativeincontrolgroup，9positiveinmildOAand7positiveinmoderateOA

4、group．111eresultofMMP-13immunohistochemistryshowedonepositiveincontrolgroup，14positiveinmildOAand2positiveinmoderateOAgroup．3．TheresultofRealTimeRT．PCRshowedthattheexpressionlevelsofBMP一2，COL—XandMMP-13weresignificantlyincreasedinmildOAgroup(6．64-，8．12一a

5、nd9．92-fold)thancontrolgroup(／'<0．01),theexpressionlevelsofthatgeneswereincreasedinmoderateOAgroup(2．21-,2．81-and2．46-fold)thancontrolgroup(／'

6、patients．2．Expressionofchondrocytematurationmarkergenes，suchasBMP-2，COL—XandMMP一13weresignificantlyincreasedinarticularcartilageofOApatients．3．TheseresultsestablishastrongassociationbetweenWnt／13-catcninsignalingandhuman0A．Keywords：Wntproteinsbeta-cateni

7、nosteoarthritiscartilage，articularbonemorphogeneticproteinscollagentypeXmatrixmetalloproteinase一13IV天津医科大学硕士学位论文目录中文摘要⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．IAbstract．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．1][【缩略语／符

8、号说明⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．．Ⅶ前言⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯。1日O吾⋯⋯⋯⋯⋯．⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．．研究现状、成果⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．．1研究目的、方法⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯21对象和方法⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．．31．1实验材料⋯⋯⋯⋯⋯⋯