pkcγ在异氟烷预处理抗大鼠局灶脑缺血再灌注损伤中的作用

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1、徐矧医学院硕士学位论文PartI．TheEffectsofIsofluranePrecOnditioningagainstCerebralIschemicReperfusionInjuryandonDistributionofPKCTinRatsAbstractobjectiveToinvestigatetheprotectiveeffectofisofluranepreconditioningagainstfocalcerebralischemia—reperfusioninjuryinratsandchangesofPKCT

2、inthefrontallobecerebralcortex．MethodsTwenty-fourmaleSDratsweighing250—3009wererandomlyallocatedintosham-operated，ischemia-reperfusion，isofluranepreconditioninggroups(藏一8)．Middlecerebralarteryocclusion(MCAO)wasperformedbysurgicalexposureofcommon，internalandexternalca

3、rotidarteriesthroughalongJitudinalincisionintheneck．AnyZonfilament(0．25—0．28mmindiameter)withroundedendwasinsertedinternalcarotidarteryandthreadedcraniallyuntilresistancewasfelt．Thedepthofinsertionwasabout17—18ram．After2hoursofischemia，theratsinischemic-reperfusiongr

4、oupwerekilledat24hourofreperfusion。Carotidarteriesoftheratsinsham-operationgroupwereexposedbutthedepthofinsertionwaslessthan9mm．Ratsinisofluranepreconditioninggroupinhaled1．5％isofluranelhourandahalfbeforeMCAOwasperformedandtheinhalationlastfor1hour．After24hoursreperf

5、usion，neurologicaldeficitoftheanimalswasassessedbyZeaLonga’sscoringsystem(O=nodeficit，4=unabletowalkandunconscious)．Theratswerethenkilled，infarctsizewasdeterminatedbyTTCstaining,andtheexpressionofPKC7infrontallobecerebralcortexWasevaluatedusingimmunofluorescence。Resu

6、ltsNeurologicaldeficitandinfarctsizeinischemia—reperfusionandisofluranepreconditioninggroupsweresignificantlyhigherthanthoseinsham—operatedgroup(P<0．oi)．111eamountofPKCTreducedinischemia—reperfusiongroup，butincreasedinisofluranepreconditioninggroupcomparedwithsham-op

7、erationgroup．AndthePKCTdistributedmainlyOVercellmembrane．Comparedwithischemia-reperfusiongroup，neurologicaldeficitandinfarctsizeoftheratssignificantlyreducedinisofluranepreconditioninggroup，buttheexpressionofPKCy4徐州医学院硕士学位论文1ncreasedinfrontallobecerebralcortex【P

8、)．ConclusionIsofluranepreconditioningiscapableofinducingneuroprotectionagainstfocalcerebralischemia-reperfusioninjury,andtheprotect