乳化异氟醚预处理对大鼠局灶性脑缺血再灌注的保护作用

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1、四川大学硕士学位论文theeffectsofemulsifiedisofluranepreconditioningofneuroproteetionagainstmiddlecerebralarteryocclusioninjury．Todetermineifemulsifiedisoflurancohasprotectiveeffectonbrainagainstfocalischemia-reperfusioninjuryinaratmiddlecerebralarteryocclusionOVICAO)model．(1)

2、Todetermineifemulsifiedisoflurancehasprotectiveeffectonbrainagainstfocalischemia-reperfusionmjuryinmts．(2)ThePossiblemechanismifemulsifiedisofluranepreconditioninginducesisehemictoleranceagainstbraininjuryproducedbyMCAOinrats．Experimentl：OBJECTIVEToprovidereasonable

3、andpracticableanimalmodelsforelinicalcerebralischemiaresearch．METHODMiddleCerebralarteryOcclusion(MCAO)werecarriedouton40maleS-Dratsbysutureocclusionviainfi'aarterymethods．Theanimalsweredividedinto5groupsrandomlyandequally(n=8)：groupA诵tll60·minMCAO，groupB讪th120·minM

4、CAO，groupCwith150-minMCAO，groupDwith180-minMCAO，groupDwithshamoperation．Theneurologicaloutcomeswereevaluatedat24hafterthereperfusion．After24hofreperfusionthebraininfarctvolumesweremeasured．RESULTSNeurologicaldysfuctionsandbraininfarctvolumesgraduallyincreasedfromAto

5、D，EgroupandA—DgroupWassignificanceinTheNDS(P<0．05)．TheinfarctsizeAgroupWaslowerthanB-Dgroup(P

6、riment2：4四川大学硕士学位论文OBJECTIVEToinvestigateifEmulsifiedisofluranepreconditioninginducesdelayedischemietoleranceagainstneuronaldamageproducedbYMCAOinmts．METHODSixtymaleSDratsweighing250～300gwereanesthetizedwitllintraperitonealIO％Chloralbydrate3．5ml／kg．Middlecerebralart

7、eryocclusion(MCAO)wasperformedbysurgicalexposureofcommon,internalandexternalcarotidarteriesthroughalongitudinalincisionintheneck．Anylonfilamentro．20·0．25mmindiameter)诵throundedendwasinsertedintointernalCarOtidcarotidarteryandthreadedcraniardlyuntilresistanceWasfelt．

8、Thedepthofinsertionwasabout18—20mm．Theanimalswererandomlydividedintosixequalgroups．shanloperationgroupinwhichearitindarteriesintoexposedbu