乳化异氟醚预处理对大鼠局灶性脑缺血再灌注损伤与p-akt活性的影响

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1、徐州医学院硕士学位论文Effectofemulsifiedisofluranepreconditioningonfocal_l‘●‘』■●l●·’1cereDraliSCnemm-rel3ertusionnn]UrvISmeoiateoDVP13l饥～ktpathway—It／AbstractobjectiveToinvestigatetheprotectiveeffectofE1preconditioningonfocalcerebralischemia-reperfusion(I瓜)injuryinrats；toapproa

2、chthemechanismofcerebralprotectionbydetectingthechangeofphosphorylationofserine

3、threonineldnaseQ—Akt)activity．PARTlMethodsFocalcerebralI／RmodelinratsWaSmadeby(MCAO)．FortyeightadultmaleSprague-Dawley(SD)ratswererandomlydividedintosixgroups(n寻8)：SHAMgroup，FRgroup，lowdo

4、seofElpreconditioninggroup(El-Lgroup)，mediumdoseofEIpreconditioninggroup(El-Mgroup)，highdoseofEIpreconditioninggroup(EI-Hgroup)，intralipid(vehicleforisoflurane)group(ILgroup)．SHAMgroupwasadministeredsaline10．5ml／kg,24hlaterseparatebloodvesselsonly，anddon’tinsertthesu

5、ture．I／Rgroup，El—Lgroup，E1一Mgroup，E1-Hgroupwereintraperitoneallyadministeredsaline10．5ml／kg,El3．5ml&酣NS7ml／kg，El7ml／kg+NS3．5ml／kg,E110．5ml／kgand30％intralipid10．5ml／kgrespectively,and24hlatersubjectedtoMCAO．Rectaltemperature，heartrate，andrespiratoryratewererecored10mi

6、nutesbeforebrainischemiaand10minutesafterI／R．After24hofreperfusion,neurologicaldeficitScOreSwereassessed．CerebralinfarctionvolumeWasinvestigatedineachgroup．Results1)After10minutescerebralI／R，rectaltemperatureandheartrateofratsincreased，andrespiratoryratedecreased(P<0

7、．05)．ThisfullydemonstratesthatthemodelWassuccessful．InadditiontoSHAMgroup，thereisnosignificantdifferencebetweenothergroupsat10minutesafterI／Rinbodytemperature，heartrate，respiratoryrate(P>0．05)．2)ComparedwithSHAMgroup，neurologicaldeficitandcerebralinfarctvolumeincreas

8、edinothergroupsC尸≮O．01)；ComparedwimI／Rgroup，neurologicaldeficitandcerebralinfarctvolumereducedinElpreconditioninggroups(．尸<0．05)；ComparedinElpreconditioninggroups，neurologicaldefcitandcerebralinfarctvolume4●徐州医学院硕士学位论文reduceddose-dependently(P<0．05)．Continuepart2witl

9、l10．5ml／kgEIpreconditioning．PART2MethodsAnotherf．o啊eightadultmaleSprague-Dawley(SD)ratswererandomlydividedintosixgroups(n=8)：SHAMgr