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ID:33004163
大小:1.18 MB
页数:30页
时间:2019-02-19
《左旋肉碱对大鼠缺血性脑损伤的神经保护作用》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、中文摘要苍白区缩小。线栓法致大鼠局灶性脑缺血模型组脑组织脑梗死体积百分比明显高于假手术组(O),LC组脑梗死体积百分比为16.47%4-4.12%,与模型组23.53%4-2.56%Lk较,差异有明显统计学意义(尸2、不同程度提高。3.LC抑制低糖低氧所致的细胞凋亡。SYTOX染色结果表明模型组凋亡细胞数明显多于正常组,模型组凋亡细胞所占正常细胞的百分率为19.50%4-3.34%,与正常组1.21%4-O.73%相比,具有明显统计学意义(尸<0.01,n_4)。200“M(LC200)和400pM(LC400)的LC预作用24h后能分别明显减少凋亡细胞百分率至2.44%4-0.93%和2.39%4-1.27%(P<0.01,n=4)。4.LC降低低糖低氧损伤PCI2细胞的MDA含量。低糖低氧损伤的PCI2细胞匀浆MDA活性为1.044-O.20nmol/mgprotein,与对照组0.494-0.10n3、mol/mgprotein相比明显升高(尸<0.05,n=6)。200肛M的LC可明显降低细胞匀浆中升高的MDA含量(O.204-0.04nmol/mgprotein),与模型组相比具有明显统计学意义(户4、01,n=6)。表明LC可增强低糖低氧损伤PCI2细胞的SOD活性。结论:LC能明显减轻大鼠大脑中动脉缺血模型脑损伤,对低糖低氧所致的PCI2细胞损伤也具有保护作用,其机制可能与增强SOD活性、降低MDA等过氧化物水平有关。中文摘要由基关键词:左旋肉碱;缺血性脑损伤:神经保护;超氧化物歧化酶;自英文摘要NeuroprotectiVeeffectsofL—carnitineinischemicbraininjuryofratsABSTRACTobjective:IschemiccerebrovasculardiseaseiSacommonandfrequentdiseasethatseriou5、slyharmthehumanhealthandhasbeenaheavyburdenbothforthecountryandtheownfamily.ttplaysanimportantroleinstudyingthepreventionandthetreatmentofthisdisease.PreviOUSstudieshaveshownthatL—camitinehasanobviousneuroprotectiveeffectsandcanbeusedinthetreatmentofischemicbraininjuryandneurodegenerativediseases,b6、utthemechanismsareyetnotknown.ThemainaimofthisstudywastoobservetheneuroprotectiveeffectsofL-carnitineinischemicbraininjuryandexplorethepossiblereasons.Methods:1.StudytheprotectiveeffectsofL.camitineonfocalcerebralischemia:WeobservedtheprotectiveeffectsofL.carnitineonfocalcerebralischemia,usinganimp7、rovedmiddlecerebralarteryocclusionmodelandcalculatingthepercentageoftheinfarctvolumeinrats.2.EvaluatethecellactivityinLC-pretreated24hischemia-hypoxiainjuriedP12cellsbvMTTassay:PC12cellswereculturedinI冲MI16
2、不同程度提高。3.LC抑制低糖低氧所致的细胞凋亡。SYTOX染色结果表明模型组凋亡细胞数明显多于正常组,模型组凋亡细胞所占正常细胞的百分率为19.50%4-3.34%,与正常组1.21%4-O.73%相比,具有明显统计学意义(尸<0.01,n_4)。200“M(LC200)和400pM(LC400)的LC预作用24h后能分别明显减少凋亡细胞百分率至2.44%4-0.93%和2.39%4-1.27%(P<0.01,n=4)。4.LC降低低糖低氧损伤PCI2细胞的MDA含量。低糖低氧损伤的PCI2细胞匀浆MDA活性为1.044-O.20nmol/mgprotein,与对照组0.494-0.10n
3、mol/mgprotein相比明显升高(尸<0.05,n=6)。200肛M的LC可明显降低细胞匀浆中升高的MDA含量(O.204-0.04nmol/mgprotein),与模型组相比具有明显统计学意义(户4、01,n=6)。表明LC可增强低糖低氧损伤PCI2细胞的SOD活性。结论:LC能明显减轻大鼠大脑中动脉缺血模型脑损伤,对低糖低氧所致的PCI2细胞损伤也具有保护作用,其机制可能与增强SOD活性、降低MDA等过氧化物水平有关。中文摘要由基关键词:左旋肉碱;缺血性脑损伤:神经保护;超氧化物歧化酶;自英文摘要NeuroprotectiVeeffectsofL—carnitineinischemicbraininjuryofratsABSTRACTobjective:IschemiccerebrovasculardiseaseiSacommonandfrequentdiseasethatseriou5、slyharmthehumanhealthandhasbeenaheavyburdenbothforthecountryandtheownfamily.ttplaysanimportantroleinstudyingthepreventionandthetreatmentofthisdisease.PreviOUSstudieshaveshownthatL—camitinehasanobviousneuroprotectiveeffectsandcanbeusedinthetreatmentofischemicbraininjuryandneurodegenerativediseases,b6、utthemechanismsareyetnotknown.ThemainaimofthisstudywastoobservetheneuroprotectiveeffectsofL-carnitineinischemicbraininjuryandexplorethepossiblereasons.Methods:1.StudytheprotectiveeffectsofL.camitineonfocalcerebralischemia:WeobservedtheprotectiveeffectsofL.carnitineonfocalcerebralischemia,usinganimp7、rovedmiddlecerebralarteryocclusionmodelandcalculatingthepercentageoftheinfarctvolumeinrats.2.EvaluatethecellactivityinLC-pretreated24hischemia-hypoxiainjuriedP12cellsbvMTTassay:PC12cellswereculturedinI冲MI16
4、01,n=6)。表明LC可增强低糖低氧损伤PCI2细胞的SOD活性。结论:LC能明显减轻大鼠大脑中动脉缺血模型脑损伤,对低糖低氧所致的PCI2细胞损伤也具有保护作用,其机制可能与增强SOD活性、降低MDA等过氧化物水平有关。中文摘要由基关键词:左旋肉碱;缺血性脑损伤:神经保护;超氧化物歧化酶;自英文摘要NeuroprotectiVeeffectsofL—carnitineinischemicbraininjuryofratsABSTRACTobjective:IschemiccerebrovasculardiseaseiSacommonandfrequentdiseasethatseriou
5、slyharmthehumanhealthandhasbeenaheavyburdenbothforthecountryandtheownfamily.ttplaysanimportantroleinstudyingthepreventionandthetreatmentofthisdisease.PreviOUSstudieshaveshownthatL—camitinehasanobviousneuroprotectiveeffectsandcanbeusedinthetreatmentofischemicbraininjuryandneurodegenerativediseases,b
6、utthemechanismsareyetnotknown.ThemainaimofthisstudywastoobservetheneuroprotectiveeffectsofL-carnitineinischemicbraininjuryandexplorethepossiblereasons.Methods:1.StudytheprotectiveeffectsofL.camitineonfocalcerebralischemia:WeobservedtheprotectiveeffectsofL.carnitineonfocalcerebralischemia,usinganimp
7、rovedmiddlecerebralarteryocclusionmodelandcalculatingthepercentageoftheinfarctvolumeinrats.2.EvaluatethecellactivityinLC-pretreated24hischemia-hypoxiainjuriedP12cellsbvMTTassay:PC12cellswereculturedinI冲MI16
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