左旋精氨酸对糖尿病大鼠的肾脏保护作用

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1、左旋精氨酸对糖尿病大鼠的肾脏保护作用作者：王丽吴晨光方春钱高静徐志刚陈艳,陈宇宁【摘要】目的：探讨左旋精氨酸(Larginine，LArg)对链脲佐菌素(streptozotocin，STZ)诱导的糖尿病大鼠肾脏保护作用及机制。方法：建立STZ诱导的糖尿病大鼠模型,随机分为糖尿病模型组(DM组)和DM4周后LArg治疗组(LArg组),并以正常组作对照。观察8周后,测定各组大鼠尿白蛋白排泄率(UAER)、血肌酐(SCr)和血尿素氮(BUN)，光镜观察肾组织形态。检测血清和肾皮质中氧化亚氮(NO)、超氧化物歧化酶(SOD)及丙二醛(MDA)含量，测定肾脏

2、线粒体膜电位及肿胀度。结果：与DM组相比,LArg组大鼠UAER，SCr，BUN显著降低(P<0.05),肾脏病理形态得到一定改善;血清和肾皮质中NO及SOD含量显著升高(P<0.01,P<0.05)，MDA显著降低(P<0.05);肾脏线粒体膜电位显著升高(P<0.05)，肿胀度趋势增强。结论：左旋精氨酸对糖尿病大鼠肾脏的保护作用可能与增加NO合成，同时保护肾脏线粒体的功能有关。【关键词】左旋精氨酸;糖尿病肾病;氧化亚氮;线粒体　　[Abstract]Objective:Toexploretherenoprotectiveef

3、fectofLarginineonstreptozotocininduceddiabeticratsanditspotentialmechanism.Methods：Diabeticmodelswereinducedby13intraperitonealinjectionofstreptozotocinSTZinr+ats.Modelswererandomlydividedintotwogroups:DMgroup(nontreateddiabeticmodelrats)andLArggroup(diabeticmodelratstreatedwith

4、Larginineafter4weeks).Thenormalnondiabeticratswereasthecontrolgroup.Theexcretionofurinaryalbuminexcretionrate(UAER),serumcreatinine(SCr)andserumureanitrogen(BUN)weredetectedafter8weeks.Morphologicalchangesofkidneywereobservedbyopticsmicroscope.Thelevelsofnitricoxide(NO)，theactivit

5、yofsuperoxidedismutase(SOD)andmalondialdehyde(MDA)inserumandrenalcortexweredetected;kidneymitochondrialmembranepotentialandmitochondrialswellingweremeasuredinthedifferentgroups.Results：ComparedwithDMgroup,theexcretionofUAER,SCrandBUNinLArggroupweresignificantlydecreased(P<0.05),

6、whiletheabnormalpathologicalchangeswereimproved;thelevelsofNOandtheactivityofSODinLArggroupwerealsosignificantlyincreased(P<0.01,P<0.05),thelevelsofMDAweresignificantlyreduced(P<0.05);thekidneymitochondrialmembranepotentialwassignificantlyelevated(P<0.05)andmitochondria

7、lswellingwasreinforced.Conclusion：Larginineprotectsthekidneysduringdiabeticmodelratspossiblyby13increasingthelevelsofNOandimprovingthefunctionofmitochondria.　　[Keywords]Larginine;diabeticnephropathy;nitricoxide;mitochondrial　　糖尿病肾病(diabeticnephropathy，DN)是糖尿病最严重的慢性并发症之一，至今其发病机制尚未完

8、全阐明。氧化应激主要由活性氧簇(rea