资源描述：
《蠲痹颗粒对胶原诱导性关节炎小鼠th17-treg细胞平衡调控作用探究》由会员上传分享，免费在线阅读，更多相关内容在工程资料-天天文库。
1、蠲痹颗粒对胶原诱导性关节炎小鼠Thl7/Treg细胞平衡调控作用探究【摘要】目的：研究蠲痹颗粒对胶原诱导性小鼠关节炎Thl7/Treg细胞平衡调控作用，doi：10.3969/j.issn.2095-4174.2014.04.008StudyontheBalancedRegulationofJuanbiGranuleonThl7/TregCellsinMicewithCollagen-inducedArthritisLIUWei-chao,LILing-yu,WANChun-ping,LIZhao-fu,ZHENG
2、Xi,QIYan,WUJing-jin,PENGJiang-yun[ABSTRACT】Objective:TostudythebalancedregulationofJuanbiGranuleonThl7/Tregcellsinmicewithcollagen-inducedarthritisinordertoexploreitsmechanisminthetreatmentofrheumatoidarthritis・Methods：Thearthritismodelswereinducedbythebovinet
3、ypeIIcollagen.20DBA/1miceweredividedequallyintotheCIAgroupandtheJuanbigranulegroupaccordingtotheclinicalscore.ThearthritisclinicalscoremethodwasusedtoobservetheseriousnessoftheonsetofarthritisinCIAmice；theflowcytometrywasusedtodetectthesubpopula.tionofthesplen
4、iclymphocytes(CD4,CD8,B220),andtheThl7,ThlcytokinesandtheexpressionofTregcellsinCD4+Tcells.Results:JuanbiGranulecouldsignificantlyreducetheclinicalscoreofCIAmicewithcollagen-inducedarthritis,buthadnosignificanteffectonthesubpopulationofthespleniclymphocytes(CD
5、4,CD8,B220).Meanwhile,itdownregulatedtheexpressionlevelofIL-17AintheCD4+TcellsofCIAmice,promotingtheexpressionofTregcellsinacertainextent.Conclusion：RegulatingthebalanceoftheThl7cellsandTregcellsanddown-regulatingtheexpressionoftheproinflammatorycytokineIL-17A
6、maybeoneofitsmechanismsinthetreatmentofrheumatoidarthritis.[Keywords]arthritis,rheumatoid；arthritis,collagen-induced；JuanbiGranule；Th17cell；regulatoryTcells；mice蠲痹颗粒是中医学家吴佩衡学术继承人、云南省名老中医吴生元教授基于扶阳理论总结出的治疗类风湿关节炎(rheumatoidarthritis,RA)的效方,主要由附片、川苇、赤芍、桂枝、麻黄、蕙该仁、五
7、加皮等组成，具有温经散寒、祛风除湿、通络止痛之功，主治寒湿痹阻之RA。最新研究表明，Treg.Th17细胞功能和分化过程具有共同之处却又相互对抗，其平衡失调影响着RA发生与转归[1-2]o1实验材料5参考文献[1]LittmanDR,RudenskyAY.Th17andregulatoryTcellsinmediatingandrestraininginflammation[J].Cell,2010,140(6)：845❷858..2]BettelliE,CarrierY,GaoW.Reciprocaldevelo
8、pmentalpathwaysforthegenerationofpathogeniceffectorTH17andregulatoryTcells[J].Nature,2006,441(7090)：235-238・[3]万春平，彭江云，李兆福，等•蠲痹颗粒对胶原诱导性小鼠关节炎的抑制作用及机制研究[J].中药材，2013,36(9)：1505-1507.[
