介素18对胶原诱导性关节炎作用的研究

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1、万方数据生堡凰遑痘堂苤查2螋生8旦笙13鲞笠8塑￡女垃j丛￡Ⅱ墅趔，丛8丛2螋，y逊：!王盟Q：g小剂量白细胞介素一l8对胶原诱导性关节炎作用的研究·535··短篇论著·代巧妹张凤山李洋；【摘要l目的研究小剂量自细胞介素(IL)一18对早晚期Ⅱ型胶原诱导性关节炎(CIA)的作用。方法重组小鼠IL-18(0．2斗引、鼠)每日1次腹腔注射发病前或发病晚期的CIA小鼠，连用5d，用关节炎评分评估关节炎病情的进展，用反转录聚合酶链反应(RT—PCR)测定CIA小鼠髌骨及邻近滑膜组织的细胞因子表达水平；用组织学染色评价膝关节的软骨和骨破坏。结果小剂量IL-18早期免疫干预CIA小鼠，不能降

2、低CIA小鼠关节炎发病率，无法延缓CIA小鼠膝关节病理学进展；滑膜和软骨附近的Thl型细胞因子和Th2型细胞因子与对照组相比差异无统计学意义。小剂量IL-18晚期免疫干预CIA小鼠，加剧了病理学进展，免疫第43天，治疗组关节炎评分(O．33x'-0．11)显著高于对照组(0．25：t-0．09)(P

3、达。【关键词】白细胞介素18；关节炎，实验性Regulationroleoflowdoseinterlukin—18inmurinecollagen—inducedarthritisDA，p洳一mei,ZHANGFeng-shan,LIYang,DepartmentofRheumatalgy,theSecondAffiliatedHospital,HarbinMedicalUnwersh$，Heilon面iang150086，ChinaCorrespondinganthor：LIYang,Email：lyan9323@hotmail．com【Abstract】0bjectiveT

4、oinvestigatetheeffectofexogenousmurineinterlukin-18(mlL-18)onearlyandestablishedmurinecollagen—inducedarthritis(CIA)．MethodsMicewereinjectedintraperitoneallywitllIL—l8(0．2R／mouse)dailyfor5daysbeforeoraftertlleonsetofCIA．Theresponsewasmonitoredvisu—allybymacroscopicscoring．Reversetranscription

5、-polymerasechainreaction(RT—PCR)wasemployedtodeterminethemRNAexpressionofeytokinesinpatellawithadjacentsynoviuminCIAmouse．Histologyofkneesynoviumw船usedto8ssesstheoccurrenceofcartilagedestructionandboneerosions．ResultsIL—l8alonehadnoeffectonmacroscopicscOre，occurrelleeofarthritis，advancementof

6、histologyonearlystageofCIA．Moreover,expressionofThleytokinesandTh2cytokinesinthesynovialtissueand‘articularcartilageremainedunchangedcomparedwiththecontrolgroup，however,apronouncedprogressionofhistologywasfoundinmicetreatedwithlL—18inestabhshedCIA．Forty—threedaysafterimmunization．themacroscop

7、icscoreinthetreatedgroup(O．33x'-0．11)wassignificantlyimprovedthaninthecontrolgroup(0．25：t-0．09)(P<0．05)．Moreover，thetuRNAlevelsofIL-10andIL-18BPbotllinthesynovialtissueandinthearticularcartilageinthetreatedgroupsdecreasedsignificantlythanthos