regⅲ和胰岛素原双基因联合干预1型糖尿病的作用及机制研究

《regⅲ和胰岛素原双基因联合干预1型糖尿病的作用及机制研究》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、分类号学号D200978110学校代码10487密级博士学位论文RegIII和胰岛素原双基因联合干预1型糖尿病的作用及机制研究学位申请人：侯文睿学科专业：药理学指导教师：向明教授答辩日期：2012.05AdissertationsubmittedtoHuazhongUniversityofScienceandTechnologyfortheDegreeofDoctorStudyontheAmeliorationofType1DiabetesMellitusbyaNakedDNAPlasmidEncodingProinsulinandPancreaticRegeneratingIII

2、proteinD.Candidate:Wen-ruiHouMajor:PharmacologySupervisor:ProfessorMingXiangHuazhongUniversityofScience&TechnologyWuhan430074,P.R.ChinaMay,2012独创性声明本人郑重声明，本学位论文是本人在导师指导下进行的研究工作及取得的研究成果的总结。尽我所知，除文中已经标明引用的内容外，本论文不包含任何其他个人或集体已经发表或撰写过的研究成果。对本文的研究做出贡献的个人和集体，均已在文中以明确方式标明。本人完全意识到本人将承担本声明引起的一切法律后果。学位论

3、文作者签名：日期：年月日学位论文版权使用授权书本学位论文作者完全了解学校有关保留、使用学位论文的规定，即：学校有权保留并向国家有关部门或机构送交论文的复印件和电子版，允许论文被查阅和借阅。本人授权华中科技大学可以将本学位论文的全部或部分内容编入有关数据库进行检索，可以采用影印、缩印或扫描等复制手段保存和汇编本学位论文。保密□，在_____年解密后适用本授权书。本论文属于不保密□。（请在以上方框内打“√”）学位论文作者签名：指导教师签名：日期：年月日日期：年月日目录摘要··························································

4、······································1ABSTRACT·······················································································4主要缩略词一览表··············································································7前言······································································

5、··························9第一部分pReg/PI双基因真核表达载体的构建与鉴定·······························15引言························································································15材料与方法···············································································17结果····························

6、····························································30讨论························································································39参考文献··················································································40第二部分pReg/PI双基因质粒的体外表达及作用研究··························

7、·····43引言························································································43材料与方法···············································································44结果·················································