氢盐水对横纹肌溶解致急性肾损伤的保护作用

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1、泰山医学院硕士学位论文氢盐水对横纹肌溶解致急性肾损伤的保护作用姓名：杨敏申请学位级别：硕士专业：内科学指导教师：高鑫2012-02氢盐水对横纹肌溶解致急性肾损伤的保护作用善篙2：嚣学删专业：肾脏内科学一导师：高鑫教授中文摘要片。血清学检查模型组血清Cr、BUN及CK、CK．MB、LDH较对照组均显著升高。模型组大鼠肾脏组织MDA、8．OH．dG、GSH．PX、IL．6、TNF．仅及ROS水平明显升高，sOD活性降低。氢盐水治疗组：肾脏形态学检查较模型明显改善，血清学检测Cr、BUN及CK、CK—MB、LDH较模型组

2、明显降低，肾脏组织MDA、8．OH—dG、GSH—PX及ROS水平降低，SOD活性升高，IL．6、TNF一0【浓度降低。结论肌肉注射甘油可诱导横纹肌溶解致急性肾损伤的产生，氧化应激和炎症反应参与了横纹肌溶解致急性肾损伤的过程。氢盐水对横纹肌溶解致急性肾损伤有保护作用，其作用机制与氢盐水的抗氧化应激及抗炎症反应有关。关键词：氢盐水；横纹肌溶解；急性肾损伤；氧化应激；炎症PRoTECTIVEEFFECToFHYDRoGENSALINEONGLYCERoLINDUCEDACUTERENALFAILUREINRATKIDN

3、EY．Postgraduate：YangMinSpeciali够：kidenymedicineTutor：Prof．GaoXinABSTRACTBackgroundRhabdomyolysisisoneofthemainreasonsthatleadingtoacutekidneyinjuw(acutekidenyinjury，AKI)．ThepathogenesisinVoIVesmultiplephysiologicalfactors，Symptomsisdif-fer，theseriouscallbelife

4、threatening．Therefore，earlyfound，earlyprevention，hasimporrtantsignificanceforrhabdomyolysisinducedacuterenalinjury．Thehi曲intensi．tymlitarytrainingandstrenuousexercisecan1eadtorhabdomy01ysis．Howtoprevent仃o．mrhabdomyolysisearly，isanimportantissuethatthepresentsp

5、onsmedicineandclinicalmedicinef．ace．PurposeInthisstudy，theratsmodelwasdeVelopedbyinjectingglycr01intothemuscleofratstosimulaterhabdomyolysisinducedAKI．Tostudyhydrogensalineexertsbeneficialef．fectonrhabdomyolysisinducedacuterenalinjurybyglycerol(10ml／l(g)inrats

6、aJldtoinvesti．gatethepotentialmechanism．MethodsWeightofabout2009ratswererandomlydiVidedintofourgroups．Thecontr01group(Contr01)，modelgroup(Model)，largedosehydrogensalinetreatmentgroup(Treated1)，10wdosehydrogensalinegroup(Treated2)．Themodelgroupratswereinjected5

7、0％91y卜ol(10ml／kg，I．M)．Treated19roupandtreated2wereglycerol(10ml／蚝，I．M)plushydrogensaline(10mg／1(g，and5mg／kgi．p．routerespectively)．Usedautomaticbiohemist．ryanalyzertomeasuresemmcreatinekinase(CK)，creatinekinaseisozymes(CK．MB)，1a—ctatedehydrogenase(LDH)，creatini

8、ne(Cr)，ureanitrogen(BUN)．andobseⅣedtherenaltissue，toassessthehydrogensalinei’sprotectiVeef．fecton91ycerol—inducedacuterenalf．ai—lureinratkidney．UsedELISAtomeasurereactiVeoxygenspec