大鼠耳蜗雪旺细胞体外培养、纯化及增殖的实验研究

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1、第四军医大学硕士学位论文大鼠耳蜗雪旺细胞体外培养、纯化及增殖的实验研究专业名称：耳鼻咽喉科学作者姓名：张心怡指导老师：邱建华论文编号：硕士学位论文大鼠耳蜗雪旺细胞体外培养、纯化及增殖的实验研究培养类别学历研究生申请学位类型学术学位学科(领域)专业耳鼻咽喉科学研究方向耳聋的基础研究二O一二年五月硕士学位论文目录缩略语表········································································································

2、·············1中文摘要·····················································································································2英文摘要·····················································································································5前言············

3、·········································································································8文献回顾·····················································································································9正文··········································

4、········································································24实验一大鼠耳蜗雪旺细胞的体外培养和纯化··········································241材料与方法·····························································································241.1实验材料···························

5、································································241.2细胞分离纯化···················································································252结果·········································································································282.

6、1雪旺细胞的形态···············································································282.2耳蜗雪旺细胞免疫荧光鉴定···························································282.3比色法检测耳蜗雪旺细胞增殖························································293讨论······························

7、···········································································29实验二耳蜗雪旺细胞增殖实验的研究······················································311材料与方法·····························································································311.1材料·················

8、··················································································311.2方法·······