姜黄素纳米微粒对体外胃癌mgc803细胞凋亡机制的影响

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1、·论著·2012年10月第9卷第29期姜黄素纳米微粒对体外胃癌MGC803细胞凋亡机制的影响潘洪明1费洪新1卢长方2陈刚2张可勇21.齐齐哈尔医学院基础医学院，黑龙江齐齐哈尔161000；2.齐齐哈尔医学院科技处，黑龙江齐齐哈尔161000[摘要]目的观察姜黄素纳米微粒对人胃癌MGC803细胞凋亡机制的影响。方法MTT法建立细胞剂量效应曲线，光镜和电镜下观察细胞形态变化，流式细胞仪分析MGC803细胞周期改变，免疫组化法检测凋亡相关Bcl-2蛋白表达。结果MTT法显示，姜黄素纳米微粒抑制胃癌MGC803细胞增殖，呈现剂量-时间依赖性；光镜观察姜黄素纳米微粒组细胞损伤较轻，电镜下核周隙

2、清晰，突触小泡较多；流式细胞仪法显示，姜黄素纳米微粒使胃癌MGC803细胞阻滞于G2/M期；免疫组织化学法显示，姜黄素纳米微粒使凋亡相关Bcl-2蛋白表达下调。结论姜黄素纳米微粒诱导人胃癌细胞MGC803凋亡并且延长药物对胃癌MGC803细胞作用时间。[关键词]姜黄素；胃癌；细胞凋亡[中图分类号]R735.2[文献标识码]A[文章编号]1673-7210（2012）10（b）-0016-04EffectofcurcuminnanoparticleonapoptosisofhumangastriccarcinomacelllinesMGC803invitro11222PANHongmi

3、ngFEIHongxinLUChangfangCHENGangZHANGKeyong1.CollegeofBasicMedicine,QiqiharMedicalUniversity,HeilongjiangProvince,Qiqihar161000,China;2.DepartmentofScienceandTechnology,QiqiharMedicalUniversity,HeilongjiangProvince,Qiqihar161000,China[Abstract]ObjectiveToobservetheeffectsofcurcuminnanoparticleon

4、apoptosisofhumangastriccarcinomacelllineMGC803invitro.MethodsMTTassaywasusedtoestablishthedose-effectcurves.Thechangesofcellswereobservedbylightmicroscopeandelectronmicroscope.FlowcytometrywasusedtoanalyzeMGC803cellcyclechanges.Immunohisto-chemicalstainingwasusedfordetectingexpressionstatusofap

5、optosis-relatedBcl-2protein.ResultsMTTresultsshowedthatcurcuminnanoparticlecouldinhibittheproliferationofgastriccarcinomacelllinesMGC803invitro,anditshoweddose-timedependence.Damageofcellwaslighterinthecurcuminnanoparticlegroup,whichwereobservedbylightmi-croscope,whileelectronmicroscopeobservat

6、ionshowedthatperinuclearspaceofMGC803cellwasclearandhadmoresynapticvesicle.FlowcytometryshowedthatcurcuminnanoparticleinhibitedMGC803cellinG2/Mphase.Immunohisto-chemicalstainingshowedthatcurcuminnanoparticlemadetheexpressionofBcl-2proteindecreaseinMGC803cell.ConclusionCurcuminnanoparticleinduce

7、stheapoptosisofhumangastriccarcinomacellMGC803,whichcanprolongthedrugactiontimeagainstgastriccarcinomaMGC803cell.[Keywords]Curcumin;Gastriccancer;Apoptosis胃癌是临床上常见的一种恶性肿瘤，严重危害人类的健姜黄素、RPMI-1640培养基、胎牛血清、MTT试剂盒、顺铂、碘康。抗肿瘤药物的研究特别是从天然植物