不同培养条件对白念珠菌芽管形成的影响

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1、兰州大学硕士学位论文THEINFLUENCEOFDIFFERENTCULTURALCONDITIOONGERMTUBEFORM姗oNINABSTRCTObjective：1．Toevaluatetheinfluenceofdifferentculturalconditions(temperature，time，pH，alternativeoxidaseanddexamethasone)totheformationofgermtubeofCandidaalbicansanddetectthegermtubequantitati

2、vely．2．TostudytheroleofSOUW：clocationinthepathogenicityCandidaalbicans．3．Tocomparewiththeincidenceofgermtubeoffluconazolesusceptiblestrains，fluconazoledose-departmentsusceptibleandresistantstrainsinthesamecondition。Method：1．Usingfetalcalfs朗皿(FCS)，inactivatedhuman∞l

3、alm，RPMIl640mediumandDMEMmedium，toinducethegerminationofCandidaalbicansunderdifferentconditions(temperature，time，pHandconcentrationofinoculum)；andcalculatetheincidenceofgermmbebythemethodofcrystalvioletstaining．2．Huconazole—susceptiblestrains，fluconazoledose—depart

4、mentsusceptibleandresistantstrainswereselectedfromthestrainsofCand／daalbicansaccordingtotheNCCLSM一27microdilutionmethod．Furthermore，calculateandcomparetheincidenceofgermtube．3．GMP(finalconcentration2mg／m1)isaddedtoCandidaalbicanssuspendedinRPMll640medium(1xl旷cell／m

5、l，pH4)，andthecellswereincubatedat37"(2for211．SHAM(finalconcentrationlmg／m1)isaddedtoCandidaalbicanssuspendedinRPMll640medium(1x105cell]ml，pH7)，andthecellswereincubatedat37℃for2h．4．ThestrainsofCand／daalbicanswereculturedonRPMIl640，whichincludediffcrcntconcentrationo

6、fdexamethasone．Results：1．Underthesamecondition，theincidenceofgeminationinFCSissignificantlyhigherthaninRPMll640mediumandDMEMmedium(尸<0．05)，andthereisriosignificantdifferencebetweenthelatertwo(P>0．05)．Thehi!ghestm兰州大学硕士学位论文incidenceofgermnationoccurredinconcentratio

7、nofinoculumofCandidaalbieansof5x106cfu／mlwiththeFCS，undertheconditionofpH7．0，37"12and2hourSofincubation；gndtheincidenceofgermnationisonadeclinewhentheconcentrationofCand／daalbicansisover5x106cf-u／m1．2．1nheincidenceofgermnationfromdifferentpartsofthesourceofCandidaa

8、lbicansstrainshadasignificantdifference(P<0．001)．Theincidenceofgermnationwashigherinthestrainsfromsystemicinfectionthanthatfromcutaneousmucosalin