“更年春”方对成骨细胞活性的影响与对er亚型、bmp2的调节

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1、●复旦大学硕士生学位论文group)．Microculturetetrozolium，p-nitrophenylphosphate，alizarinredwereusedtoobservetheeffectofgengnianchunorlpr01iferation，activityofalkalinephosphatase(ALP)andthenumberofmineralnodesofosteoblastsculturedinvitro．ResultsIndifferentGNCgroupsandEgroup，theODwoo

2、fosteoblastswashigherthanthatofNgroup(P

3、antly(P

4、iationandmineralizationofosteoblastsculturedinvitro．TheHgroupiSbetterthanEgroup．TheeffectofGNCpreventingosteoporosiSmayberelatedwithitsstimulatingosteoblastsactivities．KeywordsGengnianchunformula；osteoblast；estrogen；proliferation；differentiation：mineralizationPartIIRe

5、gulationofGNContheexpressionofERsubtypesandBMP一2inprimaryculturedratosteoblastsObjectiveToevaluatetheregulationof“Gengnianchunformula”on●Jtheexpressionofestrogenreceptor(ER)subtypesandbonemorphofleneticprotein一2(BMP-2)ofratosteoblastsinvitro．MethodsTheosteoblastswerei

6、solatedfromnewbornratcalvariaby-6．●一复日

7、大学硕士生学位论文enzymedigestion，treatedwithconditionculturemedium．DifferentconcentrationofChinesetraditionalmedicine(GNC)：high、moderateandlowlevelgroups，nilestriol(Egroup)，saline(Ngroup)andsham(Sgroup)，respectively．RealtimePCRwasperform

8、edtodeterminethemRNAlevelofERsubtypesandBMP一2．ResultsTreatedwithnilestriol。ERa、0mRNAlevelincreaseddramaticly(只0．05，respectively)．ERBmRNAlevelofdifferentGNCgroupsincreased(p

9、、MgroupsP<现0／andL、EgroupsP<口05)．ComparedwithEgroup．thelevelofBMP一2mRNAofGNCHandMgroupsincreased(P(O．01and，XO．05．respectively