rho激酶抑制剂法舒地尔对低氧性肺动脉高压大鼠干预作用的研究

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1、南京医科大学硕士学位论文respectively]changedremarkablyascomparedwiththoseintheirhypoxicmodelgroup[(19．50±0．95)mmHg，(19．75±3．41)％，(36．92±4．19)％，(60．38±4．19)％，(24．83±2．48)％，(59．34±6．12)％，(8．17±1．17)respectively(allP<0．05)]．RVI-I]wassiginificantlylower[(26．61±1．26)％】inthefasudil14dthanthatinthehypoxiagroup[(35．5

2、6±2．39)％，P<0．01]．(3)NeitherhypoxianorfasudilhadeffectonthemCAP(alI尸'0．05)．(4)Undertransmissionelectronmicroscope(TEM)，thepulmon·aryarterioleendothelialcellsinratsofhypoxicmodelgroupwasdamagedanddroppedwithmanycollagents．Themediasmoothmusclecellandhyperplasiaofcollagenfibersweresignificantlylighten

3、erinfasudilgroupthanthoseinthemodelgroup．Conclusions：Rhokinaseinhibitorfasudilcanpreventpulmonaryhypertensioninhypoxicrats，inhibitremodelingofpulmonaryartery，andobstructtheprogressivedevelopmentofHPH．PartⅡ：TheexpressionofRho／Rhokinaseoflungtissueinpulmon-aryhypertensionratsc．ausedbyhypoxiaandthein

4、terventionoffhsudil．obje甜ve--pulmonaryfasudil．TostudytheexpressionofRho／RhokinaseoflungtissueinhypertensionratscausedbyhypoxiaandtheinterventionofMethods：Seventy--twoSpraque-·Dawleymaleratswererandomlydividedintocontrolgroup，hypoxicmodelgroupandfasudil—interventiongroup．TheratmodelofHPHwasestablis

5、hedbyexposingratstonormobaficintermittenthypoxicconditions．TheexpressionlevelofRhokinaseandsmoothmusclealpha-actinfa·SMA)mRNAwereexaminedbysemi-quantitativereversetranscriptionandpolymerasechainreaction(RT-PCR)inlungtissue，respectively．ExpressionofRhokinaseproteinweredetectedbywesternblot．Thephosp

6、horylationofMBS6南京医科大学硕士学位论文(bindingsubunitofmyosinphosphatase)--asubstrateofRhokinasewasdetectedbyWesternblotanddefined．asthemarkoffimctionalactivationofthekinase．AndtherelationshipbetweentheactivationofRho／RhokinasesignalingandthedevelopmentofHPHwasclarifiedaswell．Results：TheexpressionofRhokinas

7、emRNAinhypoxicgroupwasmarkedlyupregulatedevenbeforetheonsetofHPH(the3rddayaftertheexperiment)，anditwasmuchlowerinratsoffasudilgroupthanthatofhypoxicgroup．TheexpressionpeakofRhokinasemRNAwaspriortothatofQ—SMA，andt