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1、天津医科大学硕士学位论文thepeakbloodglucose>16.8mmol/Lorbloodglucoseat120min>11.1mmol/L,whileDMhadbothofthem.After24hUMAhadstatisticalsignificancebetweenthetwogroups,OLETFratswereinDNstage.3)6ratsintwogroupswerekilledinNGT,IGT,DMandDNstages.Kidneywasweighedinordertoevaluatethedegreeo
2、fkidneyenlargement.Bothopticalmicroscopeandtransmissionelectronmicroscopywereusedtoobservetheglomerularandtubularpathologychanges.ImmunohistochemistrywasusedtotesttheexpressionchangesofMegalinandCubilinwhichrepresentthetubularreabsorptionfunction.4)Immunohistochemistrywas
3、usedtotesttheexpressionchangesofinsulinreceptorsubstrate(IRS-1),serinephosphorylationofIRS-1(pSerIRS-1),endothelialnitricoxidesynthase(eNOS),induciblenitricoxidesynthase(iNOS)andhypoxiainduciblefactor-1α(HIF-1α).Results:1)OGTT:Bloodglucosesateachpointwerenormalin8week-old
4、OLETFratsandhadnostatisticaldifferencescomparedwithLETOrats.In30week-oldOLETFrats,bloodglucoseswerehighercomparedwithLETOratsexceptFBG(P<0.05).Thepeakglucosewas18.25±4.08mmol/Land120min’sglucosewas9.56±2.06mmol/L,whichmeetthestandardofIGT.In56and65week-oldOLETFrats,allblo
5、odglucoseswerehighercomparedwiththatofLETOrats.Thepeakbloodglucoseswere18.60±3.93mmol/Land19.35±5.17mmol/Lrespectivelyand120min’sglucoseswere13.26±6.72mmol/Land13.55±6.66mmol/L,whichmeetthestandardofDM.2)Urineprotein:Therewerenostatisticaldifferencesin24hUMAbetweentheOLET
6、FratsandLETOratsinNGTstage.InIGTandDMstage,24hUMAbegantoincrease,butstillhadnostatisticaldifferences.UntiltheDNstage,24hUMAinOLETFratsweregreaterthantheLETOrats(P<0.05).3)GeneralInformation:Therehadstatisticaldifferencesinweight,FINS，TNF-α,IL-6,FFA,TG,TCandrenalindexbetwe
7、entheIGTandNGTstagesinOLETFrats(P<0.05).LevelofTNF-αandIL-6inDMratswerehigherthanthatinIGTrats(P<0.05).LevelofIL-6andTCinDNratsVI万方数据天津医科大学硕士学位论文werehigherthanthatinDMrats(P<0.05).FINSbegantodecreaseinDMratsandwaslowerthanthatinIGTrats(P<0.05).InDNrats,FINSfurtherreduced(
8、P<0.05).4)H&EstaininginIGTstage:Nobigchangeofglomerularbasementmembranethickness,glomerulusenlar
