资源描述：
《leukamenin+e对人早幼粒白血病细胞hl60生长抑制、凋亡诱导及细胞骨架的影响》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、AbstractPlantsbelongingtothegenusLabiataeIsodonarewidelydistributedinChina.Therearevarietyofcompounds,whichisisolatedfromLabiataeIsodon,applytoanti-tumortherapybasedontheirhighefficiencyandlowtoxicity.Ent-kaurenediterpenoids,whicharethemainactiveconstituentsagainsttumor,
2、arerichinthegnusIsodon.Mostofthemareusedasantitumor,antibacterial,andanti-inflammatoryherbs.Inrecentyears,itwasreprtedthatOridonin,akindofEnt-kaurenediterpenoids,havegoodcurativeeffectontreatmentofleukemia.However,thecompoundswhichhavesimilarmolecularstructureofEnt-kaure
3、nediterpenoidsagainstleukemiahaverarelyreported.Manyofthemexploretheantitumormechanismthroughtheapoptoticpathway.LeukameninEisseparatedfrom(Isodonexcisoides(SunexC.H.Hu)C.Y_WuetH.w.Li)inGansuProvince,whichisoneofent-kauranediterpenoids,thereisresearchshowsthatthiscompoun
4、dhasstrongcytotoxicity.Buttheantitumormechanismisalsonotclear.Atpresent,therearefewliteraturereporttheeffectofLeukameninEonthecytoskeleton.LeukameninE,anent-kauranediterpenoid,isusedassubjectscompoundtotesttheeffectsonHL-60cellgrowthwithtrypanblueexclusionmethodandMTT.Th
5、eeffectofLeukameninEonproliferationandapoptosisinductionwerestudiedbyFCMandAO/EB.Additionally,Hoechst33258fluorescentstainingandchromosomeaberrationtestwereusedtoevaluatetheproductionofnucleusandchromosomeaberrationinHL-60cells,respectively.Atlast,weobservethechangeofcyt
6、oskeletonwithdifferentconcentrationofLeukameninE.Theresultsasasfollows:1.LeukameninEhasstrongercytotoxicityonHL-60cells,restrainscellgrowthunderlowconcentration,andhaslethaleffectsunderhighconcentrationandatime-anddose-dependentmanners.MTTassayshowthatIC50=0.89±0.15µmol/
7、L.2.PIstainingassaysbyflowcytometrydisplayedthatthedistributionofcellcyclecouldbechangedwiththetreatmentofLeukameninE.ItcouldinducestrongSphasearrestintime-dependent.ThefragmentofDNAcouldnotoverflow.Atlast,thecellsarrestSphaseuntildead.SoLeukameninEcouldinducetheapoptosi
8、sofHL-60cells.3.TheresultsofHoechst33258fluorescentstainingshowedthatLeukameninEIIIcouldproducealargenu
