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ID:32352436
大小:1.33 MB
页数:41页
时间:2019-02-03
《apc和eb1基因表达与染色体数目异常胚胎关系》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、重庆医科大学硕士研究生学位论文T唧RELAnoNSⅢPoFAPCANDEBlGENEEXPRESIoNSWITHCHRoMoSoM哐NUM皿ERBackground:ABSTRACTTherateofchromosomenumberabnormityisabout3%inpopulation,butinspontaneousabortion,itisroughly30%.Mostofchromosomenumberabnormityembryosshowcelldivisionrestrained,e
2、vencauseembryotodie.Theattachmentbetweenthespindlesandkinetochoresprovidesessentialfunctionsforaccuratechromosomesegregation.Withthetractionofspindles,chromosomesgraduallymovetothepolesofcellsanddistributeevenlytodaughtercells.Butbynow,themolecularbasis
3、forchromosomenumberabnormityisunknown.Itwasreportedthatchromosomenumberabnormitymaybedue,atleastinpart,tomutationsintheadenomatosispolyposiscoli(APC)inacoupleofpapers.APCasatumorinhibitingfactorhasbeenwellinvestigatedinoncology.Buttheseinvestigationsmos
4、tlyfocusedon、ⅣNTsignalpathwayandfoundAPCCannegativelyregulateB—catenin,whichisaimportantcomponentofthe、7ⅢTsignalpathway.However,besidesofnegativelyregulateintheWNTsignalpathway,APCCanalsoregulatemicrotubuleassembling,stability5重庆医科大学硕士研究生学位论文andattachme
5、ntofspindlestokinetochores.Moreover,theresearchesODthestructureofAPCindicatedthatthereisastructuraldomainintheC—terminalofAPCthatcarlbindwithEnd—bindingproteinI(EB1、.Withthemanner,APCandEB1takepartinorregulatetheinteractionsofspindlesandkinetochorestoge
6、ther.EB1istheoneofmicrotubuleplus—terminal-bindingproteins,andcancombinewithmicrotubules’plus—terminalsolely.Bythismeans,EB1canmakemicrotubulesgrowtowardscentromeresandstabilizemicrotubules.Furthermore,EB1canregulatemicrotubulesbindingwithCLIPsthatarean
7、othergroupmicrotubuleend—bindingproteinsandinteractionofAPCandB—catenin.TheseinvestigationsindicatedAPCandEB1takeimportantroleintheinteractionofspindlesandcentromeres,andinmanytypesofAPCmutationtumorcellsandthecellstrainswhichAPCandEB1overexpressedbyman
8、—madeappearedabnormalcelldivisions.Asweknow,fetationandoncogenesisbehavesimilarcharacterinmanyfacets.So,itisasignificantstudytoinvestigateifAPCandEB1takecrucialroleinchromosomesabnormalembryoniccellsObjective:ToinvestigateAPCandE
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