不同途径移植人脐带间充质干细胞修复大鼠脊髓损伤的的研究

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1、天津医科大学硕士学位论文出现统计学差异(脚．05)。损伤后12周，D组和E组诱发电位SEP、MEP的P40潜伏期和波幅值与其他组间比较潜伏期短、波幅值高，差异有统计学意义p

2、B(219622．47士18944．89)、C(197995．0335士11628．49)三组，差异有统计学意义(脚．05)，同时E组较D组更小(卿．05)，而A、B、C三组间无统计学差异(矿O．05)；损伤后13周BDA神经示踪显示D、E两组轴突再生明显增加，部分皮质脊髓束再生、延伸通过损伤区域到达远端。【结论】移植hUC．MSCs能促进脊髓损伤大鼠的神经功能恢复；hUC．MSCs可经蛛网膜下腔途径到达脊髓损伤局部，并修复大鼠脊髓损伤；通过局部移植途径移植hUC．MSCs修复脊髓损伤的疗效优于经蛛网膜下腔途径移植的疗效。关键词：脐带间充质干细胞蛛网膜下腔细胞移植脊髓损伤

3、大鼠Il天津医科大学硕士学位论文Abstractobjective：Toevaluatetherepairabilityofhumanumbilicalcordderived．mesenchymalstemcells(hUC-MSCs)transplantedintospinalcord—injuredratsviasubarachnoidspacewayandintraspinalcordwaydirectly．Methods：SeventyfemaleWistarratswhichsubjectedtospinalcordinjuryatT10usingtheMASC

4、ISImpactorModel-IItypeweightdrop印paratuswereassignedintofivegroupsrandomly：plaincontrolgroup(groupA，n=lO)，DMEMsubarachnoidspacewaytransplantedgroup(groupB，n=lO)，DMEMintraspinalcordwaygroup(groupC，n_lO)，hUC—MSCssubarachnoidspacewaytransplantedgroup(groupD，n=20)，hUC-MSCsintraspinalcordwaygr

5、oup(groupE，n=20)．Afteraweek，theratsofgroupAwerenottransplantedanything，onlyreceivedSCI．FivemicroliterDMEMweretransplantedintospinalcord-injuredratsofgroupB(viasubarachnoidspaceway)andgroupC(intraspinalcordwaydirectly)．Also，fivemicroliterhUC．MSCs(1xlOo)wereisolatedandpurifiedinvitroandthen

6、transplantedintospinalcordinjuredratsofgroupD(viasubarachnoidspaceway)andgroupE(intraspinalcordwaydirectly)respectively．TherecoveriesofthelowerextremitywereobservedbyBasso-Beattie—Bresnahan(BBB)locomotorscoringsystem，footprinting，somatosensoryevokedpotential(SEP)andmotorevokedpotentials(M

7、EP)．Also，lmigration，differentiationandsurviveofhUC-MSCsintheinjurysitewereobservedbyimmunohistochemistry．TheareaofglialSCarintheinjurysitewascalculatedbyImmunostainingagainstGFAP．Thecoticospinaltract(CST)wasobservedusingthebiotinylateddextranamine(BDA)tracingalso．Re