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1、制何首乌多糖的提取纯化及抗衰老作用研究第三部分：用制何首乌多糖PRPMA、PRPMB、PRPMC生理盐水溶液对D-半乳糖衰老模型小鼠灌胃。结果表明:制何首乌多糖能较显著提高小鼠血清及脑组织中SOD活性，降低MDA含量，此外还能显著提高正常小鼠GSH－PX血清及脑组织中的活性，同时证明了其中PRPMB为主要活性部位。关键词：制何首乌；多糖；提取工艺；纯化工艺；抗衰老-2-英文摘要Studyontheextraction、purificationandaging-resistantofthepolysaccharide
2、fromRadixPolygoniMultifloriPreparataWangTing（ScienceofPharmaceuticalAnalysis）DirectedbyLiangShengwangAbstractThedryrootofpolygonummultiflorumthunb.(polygonaceae)hasflatqualities,alittlebitterandflavor.Itisgoodatdisposinghotnessandpoison,subsidingswellingandbum
3、p.whenithasbeenprepareditbecomeswarmerandsweeter,canbenefitliverandkidney,nourishtheblood,makehairdarker,andstrengthenthebonesandmuscles.Thepreparedpolygonummultiflorumisusedmoreoftenthanunprepared.Inthepaper,thepreparedpolygonummultiflorumwasusedasresearchmat
4、erialanditswater--solublepolysaccharidewasexploredinthefollowingrespects,includingextraction,contentdetermination,purification,fractionandpharmacologicalstudy,theresultswereasfollows:Thefirstpart:thepolysaccharidesfromRadixPolygoniMultifloriPreparata(PRPM)aree
5、xtractedbythemethodsofwateextracting.FactorsthathadinfluenceontheyieldofPRPMweremainlyconsidered,includingextractiontemperature,extractiontime,extractiontimes,thesolvent-solidratio,etc.Onthebasisofsinglefactorexperiments,orthogonaldesignwasadoptedtosearchtheop
6、timumextractingparameterofPRPM..Theresultshowedthatoptimumextractingparameterasfollows:90℃ofextractiontemperature,1hofextractiontime,2timesofextractiontimes，15:1ofthesolvent-solidratioandpH7.0weresuitable.Takingglucoseascontrol,thephenol-sulfuricacidmethodexam
7、iningcontentofPRPMwasestablished.Theresultsshowedthattherevisedmethodwasstableandhadgoodrepeatabilityandhighaccuracywhenthevolumeratioof5%phenoltosulfuricacidwas1:4,thevolumeratioofsulfuricacidtototalvolumeofsolutionwas2:3andreactionsolutionwascooledafterbathi
8、ng15mininboilingwater.TheaveragecontentofPRPMwas18.54%.Thesecondpart:TwomethodsofsevegandTrichloroaceticacid-N-ButanolareusedtoremoveproteinfromPRPM.Theresultsshowthatthemethodofse
