大鼠同种异体骨髓间充质干细胞移植治疗急性肝衰竭的实验的的研究

《大鼠同种异体骨髓间充质干细胞移植治疗急性肝衰竭的实验的的研究》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、摘要高其生存率。关键词：急性肝衰竭；骨髓问充质细胞；实验研究IIIAbstractABSTRACTObjective：ToevaluatethetherapeuticeffectofBMSCsontheexperimentalacuteliverfailureinRatsinducedbyCCL4．Methods：1．Selecting，separating，cultivatingandidentifyingBMSCswereobtainedfromtworatsthighboneunderasepticcondition，thencu

2、lturedandpuredbythewholemarrow-culturedmethod．Immunohistochemistryisappliedtodetectcell·surfacemarkerexpressions：CD29、CD44、CD34andCD45，whichhelptoidentifyBMSCs．2．EvaluatingthetherapeuticeffectofBMSCsontheexperimentalacuteliverfailureinRatsThirtymodelratswererandomlydivid

3、edintothreegroups：normalcontrolgroup，withoutanytreating；liverfailuregroup，withinjectionofphysiologicalsalineviacaudalvein；transplantationgroup，withBMSCstransplantationofthesamedosage，bothofwhichhadbeeninjectedwith50％CCL4mixedbyparaffinoil，1．2ml／1009，freshly—prepared，tode

4、velopacuteliverfailuremodels．Finally,survivalrates，liverfunctionandhistopathologicexaminationswereperformed，wimtheresultsofwhichtoevaluatethetherapeuticeffectofBMSCstransplantationinthetreatmentofacuteliverfailureinrats．Results：1．1mmunohistochemicalanalysisofP3BMSCsindic

5、atedCD29、CD44positiveandCD34、CD45negative，whichpresentedabrowncellmembraneforpositivemarkers．Flowcytometryshowedpositiveratesrespectively：CD29(99．3％)，CD44(99．6％)，CD34(4．3％)，CD45(17．1％)．2．Ratssurvivalrateshoweditsincreaseto70％，comparedtotheliverfailuregroup(20％)．Besides，t

6、heliverfunctionimprovedgradually,thatis，theactivityofALTandASTdecreasedremarkably．Inpathohistology,thedegenerationandIVAbstractnecrosisofhepaticcellsanddegreeofinflammationwerelessobviousthatofliverfailuregroup．Conclusions：1．BMSCs，withhigh-purityanduniformconfiguration，c

7、ouldbeobtainedbythewhole．marrowadherentculturemethod．’2．BMSCstransplantationviacaudalveiniSaneffectivemethodtotreatacuteliverfailure，increasingitssurvivalrate，improvinghepaticfunctionandalleviatethedegreeofcellnecrosis．Definitely,BMSCstransplantationcallhelptotreatexperi

8、mentalliverfailureinrats．Keywords：Acmeliverfailure；Bonemarrowmesenchymalstemcells；ExperimentalstudyV中英文