同种异体骨髓间充质干细胞移植治疗兔股骨头坏死的实验分析

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1、第三军医大学硕士学位论文StudyonEvaluatingSRYasaEngraftingTrackofBoneMarrowMesenchymalStemCellanSDIantatlonIor■JJ●^RepairingOsteonecrosisofFemoralHeadAbstractObjectiveToevaluateSRYasaengraftingtrackofthetransplantedbonemarrowmesenchymalstemcells(BMSCs)survivalandnewboneformationintheosteonecrosisofthefemoralhea

2、d．MethodsForty-nine3-month—oldNewZealandWhiterabbitswereincluded，weighing2-2．5Kg，48femaleand1male．BMSCsoftherabbitswereisolatedbydensitygradientseparationmethod，thethirdpassagecellsweremarkedbyDiI．TheanimalmodelofONFHwereestablishedwithfortyeightfemalerabbitsbyinjectingliquidnitrogen，andfemoralhead

3、wasnotdislocated，whichweredividedinto3groups，16rabbitsineachgroup，groupAonlyestablishedanimalmodelascontrol，autologousBMSCsmarkedbyDilwastransplantedintheONFHmodelsofthegroupB．AllogenicBMSCsmarkedbyDilwastransplantedinONFHmodelsofthegroupC．ThefemoralheadwereobservedbyX—ray,HEstainingandMassontrichr

4、omestainingandtheregeneratingtrabecularvolumepercentagesweredeterminedat2，4，6and8weeksafteroperationrespectively．Theexamplesoftheheart，lung，liver,spleenandkidneywereobtained．ThetransplantedBMSCsweretracedbyfluorescencemicroscope，theSRYgeneexpressionwasdetectedbypolymerasechainreaction(PCR)forcellss

5、urvival．ResultsTheX·rayshowednecrosisinthefemoralheadofgroupAgradually．HEandMassontrichromestainingresultsshowedthatcomparedwiththegroupA，therecoveryconditionofthenecroticfemoralheadinthegroupBandCWasbetter．Ateachtime·pointofgroupBandC，theregeneratingtrabecularvolumepercentageswerehigherthanthatoft

6、hegroupAsignificantly．TheresultbetweengroupBandCWasinconspicuous．CellsmarkedbyDilwerenotbeenfoundedinthetissueoftheheart，lung，liver,spleenandkidneyateachtimepoint．PCRshowedthattheexpressionofSRYgeneWasnotobservedattheheart，lung，liver,spleenandkidneyofthreegroupsateachtimepoint．TheexpressionofSRYgen

7、eWasclearlyidentifiedinthefemoralheadofall16rabbitsinthegroupBateachtimepoint．ConclusionAllograftingof2hybridizationforSRY．Keywords：Bonemarrowmesenchymalstemcells；Avascularnecrosisoffemurhead；SRYgene；Alloge