欢迎来到天天文库
浏览记录
ID:32278875
大小:5.71 MB
页数:49页
时间:2019-02-02
《大鼠同种异体骨髓间充质干细胞移植治疗急性肝衰竭的实验的的研究》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、摘要高其生存率。关键词:急性肝衰竭;骨髓问充质细胞;实验研究IIIAbstractABSTRACTObjective:ToevaluatethetherapeuticeffectofBMSCsontheexperimentalacuteliverfailureinRatsinducedbyCCL4.Methods:1.Selecting,separating,cultivatingandidentifyingBMSCswereobtainedfromtworatsthighboneunderasepticcondition,thencu
2、lturedandpuredbythewholemarrow-culturedmethod.Immunohistochemistryisappliedtodetectcell·surfacemarkerexpressions:CD29、CD44、CD34andCD45,whichhelptoidentifyBMSCs.2.EvaluatingthetherapeuticeffectofBMSCsontheexperimentalacuteliverfailureinRatsThirtymodelratswererandomlydivid
3、edintothreegroups:normalcontrolgroup,withoutanytreating;liverfailuregroup,withinjectionofphysiologicalsalineviacaudalvein;transplantationgroup,withBMSCstransplantationofthesamedosage,bothofwhichhadbeeninjectedwith50%CCL4mixedbyparaffinoil,1.2ml/1009,freshly—prepared,tode
4、velopacuteliverfailuremodels.Finally,survivalrates,liverfunctionandhistopathologicexaminationswereperformed,wimtheresultsofwhichtoevaluatethetherapeuticeffectofBMSCstransplantationinthetreatmentofacuteliverfailureinrats.Results:1.1mmunohistochemicalanalysisofP3BMSCsindic
5、atedCD29、CD44positiveandCD34、CD45negative,whichpresentedabrowncellmembraneforpositivemarkers.Flowcytometryshowedpositiveratesrespectively:CD29(99.3%),CD44(99.6%),CD34(4.3%),CD45(17.1%).2.Ratssurvivalrateshoweditsincreaseto70%,comparedtotheliverfailuregroup(20%).Besides,t
6、heliverfunctionimprovedgradually,thatis,theactivityofALTandASTdecreasedremarkably.Inpathohistology,thedegenerationandIVAbstractnecrosisofhepaticcellsanddegreeofinflammationwerelessobviousthatofliverfailuregroup.Conclusions:1.BMSCs,withhigh-purityanduniformconfiguration,c
7、ouldbeobtainedbythewhole.marrowadherentculturemethod.’2.BMSCstransplantationviacaudalveiniSaneffectivemethodtotreatacuteliverfailure,increasingitssurvivalrate,improvinghepaticfunctionandalleviatethedegreeofcellnecrosis.Definitely,BMSCstransplantationcallhelptotreatexperi
8、mentalliverfailureinrats.Keywords:Acmeliverfailure;Bonemarrowmesenchymalstemcells;ExperimentalstudyV中英文
此文档下载收益归作者所有