川芎嗪靶向内皮细胞抗动脉粥样硬化作用的研究

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1、AntiatherosclerosisinducedbyligustrazinetargetingendotheliumSPeeiality:CardiovaseularDisease，IniernalMedieineofIntegratedTCMandWestemMedieineAuthor:Guofeng认/angTutor:ProfessorChuanllua、认ngAbstraetObjeetive:1.ToexaminetheantiatheroselerotieeffieaeyindueedbyLIGanditsunderiyin

2、gmech丽sms.2.TowidenitseliniealusageseoPe，toProvidesensitivemarkersforeliniealdiagnoseintheearliertimeofatheroselerosis.Methods:1.IninvivoeXPeriments，rabbitsfed勿highcholesteroldicthavebeenusedtoevaluatetheanti毗eroselerotieemeaeyofLlo(75，一somg.kg一，)ineludingpl叫ueSurfaceareain

3、anrta，I/M，foameellamotmts，seru们nliPidlevelofTC，TG，LDL一CandHDL一C.2.F叭herinvestigationhadbeene翻edoutininvitroexPerimentsinducedbyox一LDLinHCAECthroughexami正ngtheeffeetsofLIGonthegeneandProteineXPressionofMCP一l，ICAM一l，COX一2andtheireorn们nonrelatedsignalPathwayssuehasNF一妞.3.AProt

4、eomieanalysisofLIGagainstox一LDL一indueedHCAECwasPerformed.ThediffereniiallyexPressedProteinsindueedbyLIGwereideniifiedbyusingLC一ESI一MS[MS，theSWISS一PROTdatabaseandKEGGsignalsforeeast.FurtherfunctionvalidationofthesePathwaysandtargetsinendothelialeellswereeariedout.Results:1.L

5、IGeouldredueethepl明uesurfaceareainaortaat出edoseof75andzsomg.kg一1.FMand扔ameellamountswerealsodeereased.Inaddition，serumlipidlevelofTC、TGandLDLhadbeendePressed，howeverserumlevelofHDLhadnoehanges.2.LIGmarkedlydeereasedthegeneandProteinexPressionofMCP一1，ICAM一1andCOX一2attheconce

6、ntrationofl，10and100林M.ThetransloeationofNF一忍tonuclearhadbeenrestrained.3.TwelveofaPProximately400detectedProteinswereeitherinereasedordeereasedinabundaneeasaresultoftreatmeniwithLIG.Alnongthem，9sPotsweremorethanZ一folduP一regulatedand3weremorethanZ一folddown一regulatedbyLIGeom

7、Paredwithox一LDL一treatedsamPles，identifiedbyeomP而ngmassesoftheirtryPtiePePtideswiththoseofknownProteins，usingLC一ESI·MS从5andtheSWISS一PROTdatabase.TheirfunetionshavebeenanotatedinEXPasydatabasesincludingliPidandenergymetabolism，ehaperone，reeePtortyrosinekinases，eytoskeletonreg

8、ulationandubiquitin一Proteasomesystem(UPS).Conclusion:1.Antlatheroselerotieeffeetsi