长叶红砂wrky转录因子的克隆与特性分析

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1、内蒙古大学硕士学位论文CLONINGANDCHARACTERIZATIoNoFWRKYTRANSCRIPTIONFACTORSFROMREAUMURIATRIGYNAABSTRACT尺P口“m玉旷砌们鲁咿口isanerldangeredsmallshnlbendemictotheEastemAlxa州％stemOrdosareain111IlerMongolia．T11isrecretohalophytehasdeVel叩edu11iquemo印hologicalcharacteristicsandad印tionstrategiest11atallowit

2、tocopewitllthestI．essimposedbysemi—desertsalinesoil．Duetoitssuperiortolerancetosalinity，R．护f鲁咿口isconsideredtobeexcellentcaIldidatestoiIlVestigatesalt-tolerancemechallismsandtoidenti矽e丘．ectiVesalt-responsegenesinmeplam．Asanimportant缸．anscriptionfaCtorfamily，WRKYtranscriptionfIactor

3、splayaVitalrolei11plantresponmI玛toabiotics仃esses．hlⅡlis咖d弘wefocusedonmemembersofⅥ哏KY饥aIlscriptionf配tors、Ⅳhichscreened舶m仃anscriptionlibraryofR．护这"口．Meanwhile，weanalyzeditSexpressionpattemunderaIti6cialadverses骶sses．Finally，TwoWRKYtranscriptionf-ac幻rswereisolatedf．romR．删鲁咿口，and如ncti

4、onanalysisIlI长叶红砂W鼬@转录因子的克隆及特性分析oftlletwogeneswasca盯iedout．Themainresultsareasfollows：1．67unigenesequencesofWRKY仃锄scriptionfaCtorswereobtained丘．omtr：mscriptionlibra巧ofR．驴i童咿口．Amongthem，thereare23unigenesthatsharehi曲lysimil撕tieswiththehom01090usgenesf}om脚甜，z岱fD，挖P挖改埔以．2．1Ogeneswere

5、selected舶mall67Ⅵ删“行a蛐ents．111eireXpressionpattemwasa11alyz甜undersalt，cold，droughta11dABAstress．Theresultssuggestedthat1Ogeneswereinducedbysaltandcoldstl．ess，8and7geneswereupregulatedbydrou班a11dABAtreatment．3．Twonovel黼genesn锄edRf聊Ⅸ朋and尉歹倒【砣3wereisolated行om尺．加t呈，张口byr印idanlplificati

6、onofcDNAends(RACE)cloning．SequenceanalysisreVealedthattheybelongedtotheclassIandclassIIWRKYtranscriptionfIactor'respectiVely．尺f翮Ⅸ玎shared69％simil撕tieswith彳m6砒p括砌口砌刀口么f聊Ⅸ川，andm髟例【砣3was40％identitieswith彳f≯倒(砣3．Aphylogenetictreeofthededuced锄inoacidsequencesof脓K】厂genes舶mdi骶rentpl趾tspec

7、ieswasconstmctedbyⅣ匝GA4．OsoRware．Theresultshowedthat尺f愀即wasrelatedmorecloselyto彳．砌口Z觑玎口，B榔s记口c口，，jp鲫护括a11d户幻聊绷幻阳，whileRf愀y23wasclosertoWRKYproteins矗om。％肠玎“m觑6P，琊甜职(确∞配甜垅绷，z““所andG匀肥加P研锻．4．Rf陟RKy，and尺f∥RK】厂23transcriptswereanalyzedinmreeo毽ansincludingt11eroot，shoot，aIld1eaVes．Resea

8、rchshowedthatⅣ内蒙古大学硕士学位论文eXpressi