右旋美托咪啶对脓毒症大鼠的肺保护作用与其机制的初步探讨

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1、中南大学硕士学位论文中文摘要管活性肠肽浓度无明显变化(P>O．05)，CLP+DEX组升高(P

2、e：1．Toinvestigatewhetherdexmedetomidinehasthelungprotectiveeffectbyobservingratsingeneral，broadchangesinthelungsandpathologicalchangesinHEstaining，detectionofPa02andscoringoflungdryweightratio；2．Toinvestigatewhetherthelungprotectiveeffectofdexmedetomidineisregulatedthroughupregulatingtheexpressio

3、nofvasoactiveintestinalpeptidebyassayingtheexpressionofTNF．QandMethods：gomaleratswererandomlydividedinto4groups(n=10rats)asfollowing：normalgroup(NORMgroup)，sham—operatedgroup(SHAMgroup)，cecalligationandpunctureoperationgroup(CLPgroup)，dexmedetomidinetreatedgroupaftercecalligationandpunctureoperat

4、ion(CLP+DEXgroup)．Normalgroup：noanytreatment；SHAMgroup：infusephysiologicalsalineintravenousat3．3ml／kg．hcontinuouslylhaftertheSuccessofmodeling；CLPGroup：infusephysiologicalsalineintravenousat3．3ml／kg．hcontinuouslylhaftertheSUCCESSofmodeling；CLP+DEXgroup：infusedexmedetomidineat5ug／kg．hintravenousco

5、ntinuouslylhaftertheSUCCESSofmodeling，total8hrs．Arterialbloodsamples(0．amY)weredrawn2hrs，4hrs，6hrsafterinjectionforthemeasurememofPa02．Additionalarterialbloodsamples(1．5mE)weredrawnlhr,3hrs，5hrsafterinjectionforthemeasurememofTNF．0‘andm中南大学硕士学位论文英文摘要vasoactivemtest证alpeptideaRercentrifugation；Ato

6、talamountof5．5mLofbloodwasdrawnfromeachanimalover8hrsthroughthefemoralarteryblood．ARertheratswerekilled，lungtissuesweredrawntomeasurelungdryweightratioandmakepathologicalscoreoflunginjury．Results：1．ComparedwiththeNORMgroupandSHAMgroup，Pa02ofCLPgroupandCLP+DEXgroupdecreased(P<0．05)；thelungW／D，lung

7、injuryscoreandserumconcentionofTNF—awerehigher(P<0．05)；theserufflconcentrationofVmbetweentheNORMgroup，SHAMgroupandCLPgroupmadenosignificantdifference(P>0．05)，CLP+DEXincreased(P<0．05)；2．ComparedwiththeCLPgroup，Pa02ofCLP