欢迎来到天天文库
浏览记录
ID:32219065
大小:1.48 MB
页数:40页
时间:2019-02-01
《右旋美托咪啶对脓毒症大鼠的肺保护作用与其机制的初步探讨》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、中南大学硕士学位论文中文摘要管活性肠肽浓度无明显变化(P>O.05),CLP+DEX组升高(P2、e:1.Toinvestigatewhetherdexmedetomidinehasthelungprotectiveeffectbyobservingratsingeneral,broadchangesinthelungsandpathologicalchangesinHEstaining,detectionofPa02andscoringoflungdryweightratio;2.Toinvestigatewhetherthelungprotectiveeffectofdexmedetomidineisregulatedthroughupregulatingtheexpressio3、nofvasoactiveintestinalpeptidebyassayingtheexpressionofTNF.QandMethods:gomaleratswererandomlydividedinto4groups(n=10rats)asfollowing:normalgroup(NORMgroup),sham—operatedgroup(SHAMgroup),cecalligationandpunctureoperationgroup(CLPgroup),dexmedetomidinetreatedgroupaftercecalligationandpunctureoperat4、ion(CLP+DEXgroup).Normalgroup:noanytreatment;SHAMgroup:infusephysiologicalsalineintravenousat3.3ml/kg.hcontinuouslylhaftertheSuccessofmodeling;CLPGroup:infusephysiologicalsalineintravenousat3.3ml/kg.hcontinuouslylhaftertheSUCCESSofmodeling;CLP+DEXgroup:infusedexmedetomidineat5ug/kg.hintravenousco5、ntinuouslylhaftertheSUCCESSofmodeling,total8hrs.Arterialbloodsamples(0.amY)weredrawn2hrs,4hrs,6hrsafterinjectionforthemeasurememofPa02.Additionalarterialbloodsamples(1.5mE)weredrawnlhr,3hrs,5hrsafterinjectionforthemeasurememofTNF.0‘andm中南大学硕士学位论文英文摘要vasoactivemtest证alpeptideaRercentrifugation;Ato6、talamountof5.5mLofbloodwasdrawnfromeachanimalover8hrsthroughthefemoralarteryblood.ARertheratswerekilled,lungtissuesweredrawntomeasurelungdryweightratioandmakepathologicalscoreoflunginjury.Results:1.ComparedwiththeNORMgroupandSHAMgroup,Pa02ofCLPgroupandCLP+DEXgroupdecreased(P<0.05);thelungW/D,lung7、injuryscoreandserumconcentionofTNF—awerehigher(P<0.05);theserufflconcentrationofVmbetweentheNORMgroup,SHAMgroupandCLPgroupmadenosignificantdifference(P>0.05),CLP+DEXincreased(P<0.05);2.ComparedwiththeCLPgroup,Pa02ofCLP
2、e:1.Toinvestigatewhetherdexmedetomidinehasthelungprotectiveeffectbyobservingratsingeneral,broadchangesinthelungsandpathologicalchangesinHEstaining,detectionofPa02andscoringoflungdryweightratio;2.Toinvestigatewhetherthelungprotectiveeffectofdexmedetomidineisregulatedthroughupregulatingtheexpressio
3、nofvasoactiveintestinalpeptidebyassayingtheexpressionofTNF.QandMethods:gomaleratswererandomlydividedinto4groups(n=10rats)asfollowing:normalgroup(NORMgroup),sham—operatedgroup(SHAMgroup),cecalligationandpunctureoperationgroup(CLPgroup),dexmedetomidinetreatedgroupaftercecalligationandpunctureoperat
4、ion(CLP+DEXgroup).Normalgroup:noanytreatment;SHAMgroup:infusephysiologicalsalineintravenousat3.3ml/kg.hcontinuouslylhaftertheSuccessofmodeling;CLPGroup:infusephysiologicalsalineintravenousat3.3ml/kg.hcontinuouslylhaftertheSUCCESSofmodeling;CLP+DEXgroup:infusedexmedetomidineat5ug/kg.hintravenousco
5、ntinuouslylhaftertheSUCCESSofmodeling,total8hrs.Arterialbloodsamples(0.amY)weredrawn2hrs,4hrs,6hrsafterinjectionforthemeasurememofPa02.Additionalarterialbloodsamples(1.5mE)weredrawnlhr,3hrs,5hrsafterinjectionforthemeasurememofTNF.0‘andm中南大学硕士学位论文英文摘要vasoactivemtest证alpeptideaRercentrifugation;Ato
6、talamountof5.5mLofbloodwasdrawnfromeachanimalover8hrsthroughthefemoralarteryblood.ARertheratswerekilled,lungtissuesweredrawntomeasurelungdryweightratioandmakepathologicalscoreoflunginjury.Results:1.ComparedwiththeNORMgroupandSHAMgroup,Pa02ofCLPgroupandCLP+DEXgroupdecreased(P<0.05);thelungW/D,lung
7、injuryscoreandserumconcentionofTNF—awerehigher(P<0.05);theserufflconcentrationofVmbetweentheNORMgroup,SHAMgroupandCLPgroupmadenosignificantdifference(P>0.05),CLP+DEXincreased(P<0.05);2.ComparedwiththeCLPgroup,Pa02ofCLP
此文档下载收益归作者所有