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1、英文摘要StudiesontheantioxidativeactivityandtheextractingandpurifyingtechnologyofPolysaccharidesfromCaulisPolygoniMultifloriZhangHanjuan(Pharmacochemistry)DirectedbyYangYunAbstractObjective:Theaimsofthisstudyweretostudytheantioxidativeactivityandoptimizeextr
2、actingandpurifyingtechnologyofpolysaccha-ridesinCaulisPolygoniMultiflori.ThisprovidesthebasisforthefurtherdevelopmentofCaulisPolygoniMultiflori.Methods:(1)ThescavengingeffectofSWTPonsuperoxideanion-radical(O2)frompyrogallolautoxidationreactionandhydroxyl
3、radical(-OH)fromFentonreactionwasmeasuredinvitro;Theantioxidativeactive-tyofSWTPinvivowasmeasuredthroughthekitsofSOD、GSH-Px、MDA.(2)weoptimizedthewaterextractingtechnologyofpolysaccharidesandinvestigatedtheconcentrationsofalcoholusingtheextractionrateandt
4、hecontentsofpolysaccharidesasindex.(3)Onthebasisofoptimizingoptimumalkali,wedeterminedtheoptimalconditionsofalkaliextractingtechnologywiththeNaOHconcentration(A),theextractiontemperature(B),thesolid-liquidratio(C),theextractiontime(D)asinvestigationfacto
5、rs.(4)Onthebasisofdeterminingcellulaseastheapplicableenzyme,1:10assolid-liquidratioandpH5.0asenzymetreatingpH,theoptimalenzymeextractingtechnologywasinvestigatedbyorthogonaldesign.(5)MacorPorousadsorp-tionresinwasadoptedtodecolorizationanddeproteinizatio
6、nfromSWTP.Influencefactors,suchasresintype,theconcentrationofsample,theveloci-tyofflow,theleakcurve,theelutionvolumeetal,wereinvestigated.(6)Drawthestandardcurvewithanhydrousglucoseasthereferencesubstance.Deter-minationofSWTPwasstudiedbythemethodofultrav
7、ioletspectrophoto-metry.Results:(1)WhentheconcentrationofSWTPis9mg/mL,theclearance-rateof–OHis51.7%,andtheclearancerateofO2is49.7%，andthescav-engingeffectofSWTPhascertaindosage-dependent.SWTPsignificantlyincreaseactivitiesofSODandGSH-Pxanddecreasetheleve
8、lofMDAin3首乌藤多糖抗氧化活性及提取纯化工艺研究serumandlivertissueofmice.(2)TheoptimalwaterextractingtechnologyofSWTP:aratioofsolidtoliquidas1∶25,80℃for2hand3timesofextraction,theconcentrationsofalcoholis80%.(3)Theoptimalalkaliextractingtech
