人巨细胞病毒部分抗原决定簇基因的克隆、表达与初步应用

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1、硕士学位论文Cloning，expressionandpreliminarilyuloningexPresslonandprelimina，applicationofseveralantigenicdeterminantgenesofhumancytomegalovirusName：JiangYongweiSupervisorprofessor：LiMingABSTRArTHumancytomegalovirus(HCMV)，abetaherpesvims,whichhasduplexDNAstructure，isubiquitouslydi

2、stributedinhumanpopulations．Althoughrarelypathogenicinimmunocompetentindividuals,theVirl培posesasignificantcauseofmorbidityandmortalityinorganallografi,AIDSpatients，immunosuppressionpatientsand∞OU．PregnantwomenarealsoariskgroupforthisvirusasHCMVisthemostcmmnoncauseofcongenit

3、alinfection．SiencetheinfectionswithHCMVeitherareasymptomatic01'arcaccompaniedbysymptomsnotspecificforHCMV(suchasfeverandleucopenia)，laboratorytechniquesarcthesolemeansofdiagnosingHCMVinfection．Soithassignificantsensetobuildupafast,sensitiveandspecificlaboratorymethodforthed

4、iagnosisofHCMV．thendOctolrscantaketherapeuticmeasuresintheearlyperiodoftheinfectionwhichhavepositivemeaninginaristogenesisandpreventingthevirus'sconveying．DiagnosisofHCMVinfectioncanbeobtainedbydirectdemonstrationofthevirusorviruscomponentsinpathologicalmaterialsorindirectl

5、ythroughserology．Themostdirectmethodsincludecultureofthevirusordetectionofviralcomponents，likeviralDNA,RNAandantigensinbodyfluidsortissuebiopsyspecimens．DiagnosisofprimaryHCMVinfectionisexclusivelyaccomplishedbyABSTRACTserologicalmethods．Serologicassaysarewidelyusedfordonor

6、selectionandtosupportthediagnosisofHCMVinthehostandtodeterminewhetheritisallactiveorlatentinfection．Althoughitindirectlyreflectsviralactivity，serologyprovidesacheapalternativemethodthat啪readilybeautomatedforroutineuse．HCMV-specificimmunogiobnlinM(IgM)isasensitiveandspecific

7、indicatorofprimaryHCMVinfectioninimmunocompatentsubjeets,whileitisveryoftenproducedduringviralreactivationintransplantrecipients，,AIDSpatientsandSOon．Incurrentserologicassayscomplexvirallysatesarccommonlyused．However,detectionofHCMV-spacificlgMvarieswidely，andpooragreementh

8、asbeenfoundamongtheresultsobtainedwithdifferentcommerciallyavailablekits．TheUseoft