揉髌手法对兔膝关节软骨细胞凋亡基因表达影响的实验分析

《揉髌手法对兔膝关节软骨细胞凋亡基因表达影响的实验分析》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、TheInfluenceOfTheRou-BinManipulationOnKneeJointChondrocytesGeneExpressionOfApoptosisInRabbitAbstractObjective:ToobservetheRou-BinManipulationonapoptosisofkneejointchondrocytesinrabbitexploretheinfluenceofmanipulationmechanisms.Methods:50NewZealandrabbitsdiv

2、idedrandomlyintofivegroups:ManipulationgroupdruggroupModelgroupdummy-modelgroupandthenormalgroup10rabbitsineachgroup;thefirst3groupscauseIOH-modelbyoperativedummy-modelgroupoperatewithoutdamagethecorrespondingvesselnotreatmentforthenormalgroup.17timesofRou-

3、Binmanipulationoneachrabbitinmanipulationgrouptherabbitinjectionwithsodiumhyaluronate5timesindruggroupnospecialtreatmentforotherrabbits．8weeksaftermodeledtotakealltherabbitrightkneemedialtibiaplateaucartilagetissueprocessingdetectedchondrocyteapoptoticgeneB

4、cl－2、Bax、Fasexpressionbyusingimmunohistochemicalmethodstatisticsprocessing．Results:Attheendof8weeksafterbeingmodeled,themedialtibiaplateaucartilageetissueofrightkneewastakenfortransmissionelectronmicroscopeobservation.Therewasnosignificantchangeofnucleusinm

5、anipulationgroupanddruggroup,andcollagenousfiberwasdistrebuttedinorder.Whileinmodelgroup,Karipianosiswaspresentedinmostcells,withorgazellesdisplayedunclearandcollagekenousfiberdisordered.Nosignificantchangefornucleusinshammodelgroupand4normalgroup,andcollag

6、enousfiberwasdistributedinorder.Bcl－2expressionresult(34.20±12.99)%formanipulationgroup(19.00±5.34)%formodelgroup(P＜0.05tocomparewithbothgroups);Baxexpressionresult(5.94±4.58)%formanipulationgroup(35.88±26.35)%formodelgroup(P＜0.01tocomparewithbothgroups);Fa

7、sexpressionresult(13.57±11.80)formanipulationgroup(49.31±24.32)%formodelgroup(P＜0.01tocomparewithbothgroups)．Conclusion:TheRou-Binmanipulationreducedapoptosisofkneejointchondrocytesinrabbitthroughup-regulatingBcl－2expressionanddown-regulatingBax、Fasexpressi

8、onpossiblyoneofthemechanismofdelaythedegenerationofcartilagetissueofkneejointinrabbit．Keywords：manipulation;rabbit;osteoarthritis;geneexpression;study5引言膝关节骨性关节炎(Kneeosteoarthritis，KOA)，是骨伤科常见病，多发于中老年人