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1、EffectsofroubinmanipulationonkneeosteoarthritisinrabbitsserumproteomicsmetabolismmodelABSTRACTObjective:Toobservetheeffectofthepatellakneadingmanipulationonkneeosteoarthritisinrabbitsmodelofintraosseoushypertensionserummetabonomics,willbeusing1Hnuclearmagneticmagneticre
2、sonancespectroscopy(1HNMR)technology,mechanismcharacteristicsandmanualinterventionbymetabolicdetectionanddynamicanalysisofrabbitkneeosteoarthritismodel,internallinkdiscoveryinformationandchangeassociatedwithosteoarthritisofthekneemetabolicmarkers.Methods:Juneageregardle
3、ssofsexof40NewZealandwhiterabbits,weighingabout2.5~3kg,wererandomlydividedintomanipulationgroup,modelgroup,shamgroup,thenormalgroup,with10ratsineachgroup,1weekslater,themanipulationgroupadopts"thepatellakneadingmanipulation"treatment,continuoustreatmentfor5weeks,atotalo
4、f17times.Themodelgroup,shamgroupandnormalgroupwerenotadoptanymeasurestodealwith.Aftermodelingfor1,3,5,7overtheweekend,allanimalbloodofrabbitearvein,aftercentrifugation,waitingfor1HNMRdetection.Twelfthweeksaftermodelingwasperformedinthefourgroupswererabbitearvenousairemb
5、olism,kneejointcartilage,grossmorphologicalchangesofkneearticularcartilagetissuewasobservedwiththenakedeye,thenusetherongeurtakerightkneemedialtibialplateaucartilagetissue,add4%polyFormaldehydeSolutionsealedtubesforexamination,HEstaining,toluidinebluestaining,observatio
6、nonkneejointcartilagetissueandMankin'sgradinglightmicroscopy,andthedifferencesbetweengroupswerecompared.6Collectionofserumwasdetectedby1HNMR,usingthesoftwareofSIMCA-P13(Umetrics,Umca,Sweden)wereanalyzedbyPCAandPLS-DA.Results:Grossobservation:manipulationgroupandshamgrou
7、p:articularcartilageissmooth,slightlylowtransparency,luster,noobviousdefectandnewbiological.Modelgroup:articularsurfaceroughness,cartilagesurfacecolorgrayyellow,nonormaltransparencyandgloss,obviousdefectandosteophyteformation.Normalgroup:articularsurfacesmooth,edgeneat,
8、cartilageandtransparent,shiny,nodefectandnewbiological.Underlightmicroscope:manipulationgroupandshamgroup:most
